• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00249

Gene nonA

CG4211

Information

Donor	Location	Cytology	FBti	Stock number
pM14	X:16362730..16362747	14B18	FBti0200744	81145

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	ACATCACGAAAGGGCTTTCCTGATGC	TTCAAAGGTGGGCCCAGAAATGCTG

Images

Construct Design Sequences

sgRNA sequence	TGGCTATAAGCATTATGAAACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAACCGGACTTTTCCAGGCA	AGCCATTCGTCGTCAATGTA	GGACCGACTTTGGCTCGTAT	TCTGCAGTGCCGTCATTTCA

 

Amplicon 5'

Amplicon 3'

AAACCGGACTTTTCCAGGCAACAACAATCCAAATGTAAGTTATCTAAATTCGATATAGTTGATTAGATTATCGCGGGCAATTAATTGTATACAAATATTAGACATTTTTCGGGCAACTGACCACATACATCTTTAATCAAACATATGCATGCATATAATAGCCGTTACTGATAATTGTTGCAAATATTTTATGTACAAATGCCCAAGCAAGCATATATGTGTATATACATATCGAATACTTATAAAATGAACACTTGTAGCTAAATGTGCAGGGGCAGGTACATACATACACGAGTAGAAAATATGTATGTACATATGTATAAACGAGTAAATGTTCTTGACTTTTCTAAGAGAAAAGAATTAACATTATTTATTGGGCCAATATGTATTTGTACCCACCTTAAGCTGCGTAAGTCATCAGTATTTTCCCGCTAATTAAATAATCTTTCAAAACGTTCATTTTAAACAGTAGAGTACGCATGTATTTCTATTGAACTATCACTAATTTTATGATTCCCAAGTACTTGTTTGTAATATACATATGTTTAGAGAACAGTACACATATAATTGCACATTCCTTGTTATTTGAAAAGGAAAACATTACGTTTTTATAGGAATTAGTCACTACCTACAATACATTCTGGTAAATTATAGTGCTCAGTGAAGCAATTAAATGGTTTGCTTATTGATTACATTGACGACGAATGGCT

GGACCGACTTTGGCTCGTATAGTGGACTGCGTTTCAAAGTATCGCGTGCTTGAACTACAAATACTAAAATTAATCAAATTGCTTAGCTTAGTCTATAACCTGTTTACCCTGGCACTTATAGTACAAAATGTTTCGGATAATTGGTGCCAGATAGTTTTCATAACATCCAAGGGATGGTTTTTGAATTGCCTAGAAACTACAGATGTTTTGGAAAACCGAAACTAGATTCCAATGACTTCTATCCTTTTGCTGCTAGAAGAAACTTAACTTAAGTGTACTTTGCTGAGTGGATGTATCAAGGACAAACCCGTTTTCCAAACAACTAATATTGAGTACTGCACTGTAAGGCTTAATGAATTTATATATAATCTTTAAAGGGATGAAAAGAATGTTTATCTCATTAGAACAAAGACCGTATTTAAATTCACTTCTAGTTGTCAATTTATATAGGGGAAGTTAAATAAGGGAGGCACGCCTGTAGAGCCGATTCAATCGATATCAGTTCAGTTTGTATTCAAATGGTGTAACTGAATTGAAATGACGGCACTGCAGA

 

---

Search Again