CR00254

CRIMIC CR00254

Gene CG1544

CG1544

Information

Donor Location Cytology FBti Stock number
pM143R:31197808..31197829100B5Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK no band GGCCAACGAACCTGTTGCATTATCAGC AAAAAGGATTCACGTGCACATCGCCC

Images

Construct Design Sequences

sgRNA sequence TAATTAGTGCACTGCACGGGGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCGTCGAGATAACCATCGCA GGGGTCTGCCACAATCTCC GTGAAGAGAGGCGGATGTCC TTTGCCAGTTAGACCGACCC
 
Amplicon 5' Amplicon 3'
CCGTCGAGATAACCATCGCATCGATAAGAGTCTTTCGGGATCAAACATTCATATGAATGGGCAGTGGAAATCTACCCAAGCAAAAAGAAAAACCGACGAAGTGACATTGTGTGCGTGTGTGTGCGTTCGTGTCGGATTTTCCAGCCGGCTATTTATCAGCGGAATTCAAGCAGCTGACCCACAACAATCCCCGCAGAAACTGCAATAAAACAACAGCCTGAACAAGAGATCAAGTGCAAATTGCTGTGGGTGAATTCGAAAACCAGTTTAAAGAAGATATATCATTTGTATACCCTGTAATCGGAGGGTATATACGGGAATACGTATGGATACCTAGGATTATTTTCACTAACTTCAGGTTTAACTTAATTTCGCAAAATATTTTTTAAATTGAAGTTCATTGTTTATGCCTCTCATACTATCGAGAAAGAACAGCTCCTGTTGACTGGTGGAATATTTTTTATGGTTTTAATGTTACCGTCTTTCCTGTTACCTACCCAAGCTAATCTCCACGTTCTCAGCTTATCACATGTTTTCACTCTGAAATCTGATAAACCCGAAATATATGGCGCTACACCCAGTACCCCGAATTTATAATTAGAATGAGGAGATTGTGGCAGACCCC GTGAAGAGAGGCGGATGTCCAGTGCAGAGATACTATATCTTTTCCCAAAACGCACGGCAGGGAGTCCAAAAAGGACCCGGCTAAATATCAAAGCCCGGCAAACAAAGGACCCCAAAACGGGAGCTTCTTTGTGCTTGTCCTGCATCCGTTGCGTACACAATTGGGGCTTATCGACACCATGGCCAACAGACACCAGTCACCAGTTGGTAGTTGGTAGTTGGATAGTCTGCCAGGAGCCTTTGACTGGGCACCAGAGGAAAGCCTTCAACGCTTGACCATGTGACATCAGGCGCCTAGGATGATGCACATAGAGAAAATCAAGTCTAGGATTTACTCAGGCTTTGGAAAAGTATGTTAGATATTCTAAAGCAAACCCAATTGACCAGTATTTTGAATTATGGTAAAGCGTACTAAAACTGACTTTAAGCGTTAGTTTTGCCTCAGTGTAGTATCGGTATAATTCGCCTTGTTTGCCGTTCGTCCTTTGGCTGCATTTCTGTCAGGGCAATCATTATCCTTGGCAAAGGACGCGCCGCTGTGCTCCAATAATATTTACACGCTCAAGCTGAAATGTTGTCACCGCAAATACAATTCATCATACACCCTGTGCTCCTGTCCAAGCGACCTACTCTTTAACATTAAATTGATTTATGGCCGAAATGCCGTCGAGAAATTGCATAAATTTCTGAGGACGACTTGGGGTCGGTCTAACTGGCAAA
 
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