CR00257

CRIMIC CR00257

Gene Syn1

CG7152

Information

Donor Location Cytology FBti Stock number
pM143L:21769747..2176975578F1Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band no band GGCACAGAAATGGAGCGGGGTAAATT TGGGATCCTTGCAAGCTACGCACTA

Images

Construct Design Sequences

sgRNA sequence ATTTACTACGCGCGCTTTGCCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCTTGTGGCTCTGTAATGGC AAGCTCCGTCCCTGCGAC AAGCGCGCGTAGTAAATGTG TGAATCTTACCCGCGTTGCT
 
Amplicon 5' Amplicon 3'
GCTTGTGGCTCTGTAATGGCATTCTTTCTTCTGTCAGTTATCAGCTGTTACTTTTAGCTTGCTTTAGAAAAAAAGTGCGCGAAATTTTGTCCCCTACCCAAGTACTCCCCTTACGATAATGTTTCCCTCCATAAAATTTAATTATTGTCCACTAGGACAATAAAATAATAAATAAAAGGCTACACCAAAAAAAAAAATAAATTTTGTTTACATTTGTTTGTTTGGCGTCAATTTTAATATGGAGCATTTTCGTCATAAATATTTAAAATATTAAATTGAATTTCTAGTAATTTTGTAGTAGTATAGCCTTTACTTCGTTCCTTTCAACGAACAATATATTTGTTATTTATTGTTATATTTTTCTTCCTACTACACTACTTGGACATGAAATTAAATCACAACTTCCTTATGACTTGGTAATTCATTTATAAGAAGGTCCGTCACCTTATATCTTTATCTACATATGTATGTACATATGCCAAAGACGCTAGAATAAGTGTCTTTGCATTCTTTTTGATTCTTTTCCATAATGATGAAGTACGATTTTTCAAGTTTCACTTAGTGGTTTTATTATAATGGGTCTTATGAGTGCAGTAAGCACATTTTACGCGAAAACCCTAAAGACAAATATTGTACTCCAAAATGGCATAAATTATATCAATTTAGAACCCAAACACAGTATGCAAATGAAATTTCAGGGTCTAATTCTTTCAGCCCGCGATAGCAACACTTTAAACTCAAGATAATGAAAATTGGGCTTTGCTGCATGCTCTCTTCGTAATAGTAATCTCCACCCCTCGGAACCGCACACACCCACACTTTTAAAGGGAAAGGAAGAGAGCAAATGGCAACAACAAATGATAGCAGTGGTTATCTGTCAACACATATCGGCAAATGCATGTCAATTACCGCTTAAATGCTTAGCACAAAAAGCCGGCAAAAAGTGCGGAGTATGGGTGGTTTGAGTAGGTGGGTGGCGATGATGATGAATGCCGATAGTAAACGGAATTAACTTTCACGCAGCATAACGGCAACGAATGCAGCAGCGGCGACTTGAATGAAAGAAAAAGCTTACGCCGTCGCAGGGACGGAGCTT AAGCGCGCGTAGTAAATGTGCGGGTGGTGTTTTGGGGGAGGAGCGCGGTATAAGCCATCGATGCGTGGGTGCACACTCTTTGTTGACAATAAAACATATCCAAGAAAATCCACAAAAATACTCCGAAAAATGAAAATAGCGAAGCAAACCAGTAAACACCTACCATTCGATCGTATTGCGAAGTACTTTGCGAAAAGCAATTAATGCTCAGAAACTCGAATTGGCGCCTGCGCGTAAACACACACCTACACTCACTGACACACACTGTGGCGCTCCCGTGATCCAGTGCGTGCGTGTGTATTAGGTATTTTTTGCTTTTATTATTTTTTATTATTATTTTATTTATTTTTTTTTTTATTTTTTGATTTTATTACACTACGCGGCGGCTACCCTTTTTTCAGAGTCCAGAAAACCTGGTCAATTGACATGCAAACATTTACGCGATGCGACGTTGGCCCAAGTCAACACTTAACATTAACAAATGCCTTACTACAATACTGGTCGGCCAATTTGTTCTTTGCTTTTTGTCTGGCGCTTTTCATTATTATTGTTTGTTTGGCGGCGACGGCGGCGCCCGTAAAAAGCCGTTAGAAAAGCGGGAAGCTGAATTCGTTGGCGTTGGTAAGTCGCGAAGCAAAAAAACGTACGAAAACGCACTTTTATTTTAAATAAGATATGCGTGTTGTTTTACCCCGCAATGTATCCGATATAGACCACTTCCGCTGTATTTTTCAAATATTCGCAGAGGGAACCAATAGCAACGCGGGTAAGATTCA
 
Search Again