CR00261

CRIMIC CR00261

Gene whd

CG12891

Information

Donor Location Cytology FBti Stock number
pM142R:10470793..1047080047A9FBti020075081147

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK TTTCCATGGCCAACAACGGACCGAA CAGGCCTGGAAACCGATCGTATCAT

Images

Construct Design Sequences

sgRNA sequence CATTGTGGGGAGACGGTTTGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGCTTCGGATGGTCCTTCAT CCGTCTCCCCACAATGCTAA GGCAAATTTATGGCAAGCAACG GTGGGAGAACCTGATCGTCG
 
Amplicon 5' Amplicon 3'
GGCTTCGGATGGTCCTTCATGTGCTGCTCGAACATGCTGCGAATGTCTGACAGAGCCTGCGATATGTTCTGAGCGAAGCGGTGCACATCCTGTAAAGAAAACAATTCGATTAGCTGTGCTCCATGCCATGAATCAGGGCTCCTCATTACCGTTTGCTGGCACGTAGTCTTCGCTGAGATATGGAAGAATATCAGGTTCTCTCCGGCAATAATGTACGACACACCGTAACCATCATCGGCCACGGGCCCAAAGCCGCCTCCAGCGCTGATGCAGTTCGGATGCTTCTTCAGGTCCATCTTGGGCGTCTGGCCGTGCGGAGTCTGACTAGTAGAGAGGCGCCAGGGTTCGCTGAGCACCTCGTTGAGGAAGGGTGAATCCACCTCCAGGTACTTGGAGACCACATACAGGCAGAACAGATGCCTGTCGATGCCACGACCGCACATGGCATCCTGGTAGCCCAACTGGTGACGATCGCAGGCAGCCTGCATCATCTTCACACGCTCATCGTTCTGTACAGCATGGATATATGGATTAGCCTTTGATCTAATTACATAAAATTGTAAAATCTTACGGTTGTATTTGGGTTCTGCATGGCCTTGACCCAGGCGGATGACTCAATGGTGCACGGACGCACTGTCTCAGTTCTTCCTTCGCGGAACAGACGGGTCATTGAAGCCTCATATGTCAACGAGAAGCGACCGGCATCTCGGTAGTAGGCCAACTGCAGGGCCATTTGTATGTAGGCATCTGGTGAGATGCGGCACTTCTTCATAAAGCCCTTGCCGTAATCCTGGTGCACCAGGATGCGCAGATTGACCTCGTTGATAAGCTTCGTCACATCGATGGTGGCCTCTTCGATCTGCGCCAGGCATGGTTTAAGATCCCAAGTGAGCCTAGTGGGCGTGGGTGGCTGGAACGCCGGGGTGCCCTTTGTATTACCCGTTTCGTCATATCTACAGGGTTACCAAGGGAATAGGTTAGCATTGTGGGGAGACGG GGCAAATTTATGGCAAGCAACGTAAATCAAAGTATTGTTAATTTTGTGTAATTGTTGTTAGTCACGAAAACAAAATAAACTAAATGTTTACTTTTGGGTTGATTACAATTTGTAAATACAGTAAATAAAGACAGTAAGGAAAGTAAGGAAAAACAACTGAAATGGCCAACATAAGCTTATATAAAATATGCTGCGTACTGATCAAACTATTCCTCATGTAATAAATTAATAATCCCTTAAAACGTGTCTGGCATTCGCATACTAGATTTTGTATGTTTCAAGTTCGTTATGCTAGTATTTACTGTATGGCGAGATCATTAAAGCTTTTAGCGCCTAGTGATCTCTCGTACTCAACCCAAAAATGACTTGAATAACTTTGCTTTGTGCTTGTGTGCATTGTAGTTAAGTGTTTACCCATAAATATCATCACCAAATATATACTCCCACAGGTGTCCCAAAACCGGTGCATCGGCCCTATAAAAAAAGTGTAGATGGTGTAAATAAATTGTTAGGCCACAACCGGAGTTAATAAAAATAATAATAATTTTTGATTGGGACAATGGGTGATAGGTGATGATAACTAAACGCAGCCAACCGAAACCGAAAAATTGCAGGCGGTATAGGAATCGAAAATCATTACCCGTCCGATACAAGATCATCGACGATCAGGTTCTCCCAC
 
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