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CRIMIC CR00268

Gene shf

CG3135

Information

Donor	Location	Cytology	FBti	Stock number
pM14	X:6772926..6772952	6C13	FBti0199479	78886

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTAATCCCATCGGAGGTCAACTACGTT	CAGTGGCTCACAACTGACCCTCGAAA

Images

Construct Design Sequences

sgRNA sequence	AGCCTAGATCTTCTTCGAGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGTTCTTGCAGGGGATCAC	GCAATAGCCGTCTTGCAGC	ACAGTTGGGCCCCCTGTATA	CCGATTCCATTACCCCACGT

 

Amplicon 5'

Amplicon 3'

TCGTTCTTGCAGGGGATCACACACTTGGCTGTGAGGGAAATGGGTGGGCAAAGGTTCAGTGTTTCGCCTCGATAAGAGTTGAGCCGTTTGCTACTCACAGTACTCGCAACGCAGGCCGTAGTAACCCTTGGAGCACTGGCACTTGTCCTTCTGTATACACTTGCCGCCATTCAAGCACTTGTCCTTGCAAATACCTGTTGATTACAATGGGATACTATTAGTTGCGCGCCAGGCACATTTCATTCTTATATAATGCCCAGTTATCTTGCCCCAAGTGTACTTAATCATCGCGTATCGTATCACCCTCCTTTTCTACGCAGATGGAATGCTGAGAAGAGGTCTTAGCCGGCCCGAGCCACGCCTGCTCTCCAAGCGCTATCTAAGCTCAGCAACTGGAGGAGGATCAGCAGGCACGACCGAAGGAGGCAGCTCTGAAACCACCCCGCTGCGTCCACGTCCAAATGCGCTCACAGATGAGATTATACGCGTCGATCATGCCGGCGAATTGGGAGCAGATCGCATTTACGCCGGGCAAATGGCCATTCTGGGCAACGGACCGCTGGGCAAGACCATCGGACACATGTGGGAGCAGGAGAAAGAGCATCGTAAACAGTTCGAGCAGCTCATTCAACAGCACCGCGTGCGTCCAACGATCATGACGCCAATTTGGAACGTGGCCGGATTTGTGCTGGGCGCCGGAACCGCCCTCATGGGCGAAAAGGCAGCCATGGCCTGCACGGTGGCAGTGGAGACGGTGATCGTAGAACACTACAATGACCAGCTGCGTCAGATAATGGAGGCACCGAACCCGGATAAGGTAAGTACCATTTGCAACTGCCCCAAGCAACGGACGAAGTAACAACGTTATATGATTTTGCAGGAGCTGTTGGCCACCATCACCAAGTTCCGGGACGAGGAGCAGGAGCACCACGACACGGGCATTGATCACGGTGCCGAGCAAGCGCCCTTTTACCAGGCCATGACCGAGGTAATCAAGTTTGGCTGCAAGACGGCTATTGC

ACAGTTGGGCCCCCTGTATATATCAAAATCAAATTATCTGTTCCGCTAAGCTAACCGTGTTAAGCATTAAAGATAGAGCTAAATGTCCATAATAGTCGCGTCTACTTACCACCTTCGCACTGCGTGCCCTGGTAGCCCTCCGGACAGGTGCAGACGGACGGCGCCGTGCAGTTACCGCCATTGAGACACTGCGGAAAACAGAAGGCCGTCTCGCAGTATTGGCCCGTGTAGCCCACGTTGCACTTGCAGATGTGGTGCTCGTTGCAGTACCCGTTCTTGCCGCACTTCAAGCTGCATTCTGGATCGGGAGCTTAAGTTTTTTTAGTTTAGTTCAATCGTTCGCATGTCACGAGGCATAAACACTCATTTTAACACTCATTTGGACTCACACTTAACGGAGCACATCGGAAGCACAAAGAGGGGCAGGTTCGAGTAGACAGTGGATACATAGATCATAGATAGATAGATACAAAAGCGGTTGGCGTGGGAGCAAAGGGTAGATCGTATCGAATTATATATTTTTTGCGGGCTATCAATCAATAATTTACTATCGAAAAATTTGGAACAAATTTGAAGAATTTTCGAATTCAAGAATGAATGCAATTAAATTTACAATTTCATATAACATATCTCATAAAGGCTATATAGTTCCTTGTGACTTGAATATGTGATAATGTCATTAGGTAGTTACTTTCGCCAGATTCTCTTTGATAGCCCGAGTAATTTAGTGCTGTGGAAAGAGTGAAGAGCTGGGTGCAGGGGACTCGTGAAAAGCGACTACGTGGGGTAATGGAATCGG

 

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