| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| pTGEM | 3L:1780601..1780625 | 62B4 | FBti0206940 | 86508 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | short band | TGGACAACTCGGCCTATCTGCAGTTCAT | TTAGTTCTGGAACAGCGTTCGCGTGAAG |
 |
| sgRNA sequence | ATATACACTCAGATCGGGATCGG |
| sgRNA2 sequence |