| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| pTGEM | 2R:8954224..8954251 | 44F12 | FBti0206941 | 83164 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | ATGTTATCTGCGCTCCGGCTAATCTTCC | GCTGATGCTGCTACTGCTGCATCTGATT |
 |  |
| sgRNA sequence | TGTGCCCGGCCAGGTGCCCCGGG |
| sgRNA2 sequence |