| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| pTGEM | 3R:8717443..8717460 | 85A8 | FBti0206942 | 83165 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | no band | GCCACATTTGTGCATGTGCGTTTGAG | AACGCCACAAAGGCCGGGCAAAAGAAT |
 |
| sgRNA sequence | GGTAGGGGGATGGCATGAAATGG |
| sgRNA2 sequence |