| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| pTGEM | X:1924027..1924034 | 2B16 | FBti0206944 | 83167 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| short band | short band | TAATTGCATCATCCTGCCAGCTGCCG | TGTCCATCATCTTATCGCTGCGGGA |
 |
| sgRNA sequence | CGAAGACGTGAGTGTGATTTAGG |
| sgRNA2 sequence |