CR00280

CRIMIC CR00280

Gene Surf4

CG6202

Information

Donor Location Cytology FBti Stock number
pM37-p03R:15345439..1534545088F1FBti019955679231

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band short band GCCTATGGGCCCACTAATTGTTCAG GCGCGAATGGATTTGGAGTTGGGTT

Images

Construct Design Sequences

sgRNA sequence AGGTCGAGTTCCGTAATAGTTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AACTTGCCATACGCTCAGCT AGAAAGCCAGGTGAACTTGCT TTACGGAACTCGACCTGGCC TAGGCCCCATCACGGCTATA
 
Amplicon 5' Amplicon 3'
AACTTGCCATACGCTCAGCTCAAAGCGGATCAAAGTGATGAACATGAAGGCCAGCAGGATGCGGCCCGCCAGCTGCATGAAGTTCTTGGGCTTGTTTTCGCCCATCGATGGCACGCCGGCAAACAGGCTGCGCCCCTCTATCCTGGCTTCCGCCAGGACGAGCAGTAGGGCTCCGATGAGTGCAAAGTTTCGCAGTAAGAACTGGAAGTCCCACAGAATGGAGTAGGCAACGGTCTGCAAACAGAGGATCGGGTTAACGTCAAGGCTTTTGCTTCTGGCTGATATAAATGCAGACTCACCTGCAACACCACAATGAAGAAAAGCAGTCCCACCGCTATGTCGACCTTAAATCTGGCCATCACCATGCCGCAGCCGCCCAGTTGGCCCAACAGGTTGACGAGCACAAAGACGGTGGCCAGGAACTTGCCGCAGCCCCAGCTCATGTCCATGTACTCCCGCTGCTCGTTCCATTGGATGTACATGCGCAGGCCGTCCTCGAAGAACGTGGCGATGAGGCACAAACGGGCCACTGTGGGCAGCACATTTTTGCCGCGCTTGATGACCTGCAAAAATGAGTGTTCGATTGGTTCTGGTTGAGTTGAGAGCAATTGGGATGGGTGCTGATATGGTGATAAGGGGACTACTGACAAGGTGATAAGGGATTTAAATATACAATGCCGTTGAGCGTGGCTTGCGCGTAGTCGCTAGAAATCGCCCGCTGGCTGTGACGTGTCTCTAAACGCTAGTATCTCTCGATTGCTATCTGCCAGCTAACGGGTTTTGGCCCGGCTGTCAATGTAACTTCATACATGTATGTATGTAAATCGCGAGCAAGTTCACCTGGCTTTCT TTACGGAACTCGACCTGGCCGCTGGCGTCCAGCTGCCCGCTATTTCTTTTTCAACACTTTCACTTGCGGAGACAGCTCTCTCGCACACACACACAGACACATACGCATCAGCACACAGGTAGAAAATAAGCAGCGAGAGAGAGAGTTTTCCCTGCGGCAGACGCAACAACTGTCAATTGCGGTACTGGAGTACAAGGCAAACCCATCATCTGGGTTGCACCCAGATTCTTATTGGAAGCAAATGCCCAGCACGTTGCCCCCATGTCAGCGGATCGTGATTATATGCAGCCAAGACCCTCGAACTTTGCCGCGTTTGGGTGACGTTGGCAGTTTTCCCAGTTTGCAGCTGCCAGGGCTTCCCCGCAAACGCCATTTGGGGCGCATCGGGAAGACGCTTCCTGCGGTGGATATGGAGTCCTGGTGGACTTCTTTTTGGCAGTGGAACCTAATATATCTTACCTGTTCCGCCACATCCTCCGTTTTCGCTATGTACTCGTTGGGTATGCTCATGTTGGCTGCTTTGGGCTGATCCGATCTCTCACTTTGGGGCAATCGTCTGTGGAGACACTTTGGCAATTCGTTTCTGACTTCTTGGGCGTCGCGTCGCTCTCCAAATTGTTGTTAAGTTACCAAAAAATCATGAATTAAAAGGCAGGAACTTAAAAACACACACGCACGACAAAGTATAGCCGTGATGGGGCCTA
 
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