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CRIMIC CR00284

Gene CG12766

CG12766

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:3939943..3939952	64A1	FBti0199558	79233

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CGAGTTTTGAGCTGGAAAGCAGGAGCAA	GTCCAAGATGGCATGATCTTCAGCATCC

Images

Construct Design Sequences

sgRNA sequence	ACGTTAACCCGGCTACTGATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCCGATGCTCTTGGTCAGG	GCCGGGTTAACGTCATTTGA	GGCGACATTCCTGATCCGT	TAACCGAATCAGCTTTGCGC

 

Amplicon 5'

Amplicon 3'

CTCCGATGCTCTTGGTCAGGCCCAGGTCAACCAACTTCTCCATGGCGCCCCAAGTGTCCAGGTAATCAATGTCTCTGTAGTTTGAAGCAAGTAAACGGCATAGTAAGATTACTGATAATAGAAGGCGCATTTCACAATCACTTGTAAGTGCCAATCGATTCGAAATATTTCATATATTATTCATTTTACTCACACAAGTTCCACCTCTCCATTGGCATCTTTGGGAATCAGTTCGTTATCACCGCGATATTTATAGGAGAAAGGCCAGTGAATCAGATAGAGGTCAACATAATCCAGGCCAATATTCTTCAAGGTCTTTCGGCAGGCATACTCCACCCTTTCCGGTTCATGGAAATTGCACCAGAGCTGCGGAAATATGTGGCCACTTAAGGATTAGTTTATGATATGCCTGCTATAAGAACTTGCCAACTGACCTTGGTGGTGATGAATATGTCCTCCCGCTTGATGACGCCTTCGGCAATCTTCTTCCGAACCGCCGCACCCACTTCGGCCTCATTTCCGTAGAAGTAGGCGGTGTCTATGTGCCGGTAGCCCACATCAATCGCGTGGAGAACTGCCCGTTCGCAATCGCCTTCCGTCGACTGAAATCACAAGTCATCAATAACGTTAGCGGTTCTTCAAATGACGTTAACCCGGC

GGCGACATTCCTGATCCGTATCGGATATGCTATGATCAACCAGTCAATGTCATACCGCGAAGGTGCCCAGTCCGATTCCCTGGATATGGGTTCCGTCGTTGTGCTTGAAGTAGTACTCCACTTTCCCGGTCATCTTCGCGGTTACAAAGTTGAACACTGAATGCTTGGGTTGCTCGCTTCACCTCTGTTATAAGGTTTTCGAATGGGCCCATAGAACCTTCAAAAGACCTTTTGCATTTGCAGATAAAACACGGCCAAGCTGGCAGAGAAACTTGGAAATAGTTTATAGAGACGCAAGGAGCGTAGGTTCTAAGTGTGCTAACCAATATATTAAATGCATTATTTTAATGTTTTAATAAGGATAAATTAAACACATATTTTTTATAAATGATCCAAACCAAATCATGTGTAAAATCCCATTTAAAAATATGTGGGAACACTTTTGAAAAACACTTATCGATAGCTTGATAGTTGAGTGGCGCGCAAAGCTGATTCGGTTA

 

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