CR00286

CRIMIC CR00286

Gene ADPS

CG10253

Information

Donor Location Cytology FBti Stock number
pM37-p02R:14931066..1493106751D3FBti019955979234

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band short band CGGTCGATGTGAGTGCGATATGGTGATA AGGGATCCGTAACGCCTCAACGAT

Images

Construct Design Sequences

sgRNA sequence TTATGACAATGTGCTATTTCCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CAAGCCGCAAATGACTTCGA TCCGGCTTTACGTTTCCCTA AAACCGGAAATAGCACATTGTC TGGATTACTCTGCGGAGGGA
 
Amplicon 5' Amplicon 3'
CAAGCCGCAAATGACTTCGATGAGCAGATGAGAAGACAATGATCAGCATTTCGTATTTGGTCTGCAGCCGGGACACGCACACGCAAATCAGTTGTTGTCTATGGCCATTGCTACACAGCAAGAAATAGGTATTCAAAATTGTAAAGAAGAAATGCTTCAAGAGAAATACTCCATTTATTTATCAAAACCCATAGAAAACATGAGCCCGCTACAAAAGAACTGGAGACCCGAACCCCCAAGAGCGACTAGGAAAATCAGTAAAGTGACTAAGACTAGTAAACGAACTAAGCGCACTATCTAGTTGGCTACCCTCTACAATTTGGCCTTTGGTGGTGTCGATGATGTGGGTGGTGGCGGAGAAGCCTGAGCCTCCTCCAGGGGCAGAAGGTTACCGAGAGCAAAGATATTCTTCGGATCGAGATGCCGCTTGGCCGCCGAATATAGTGAACTGCCCGTTTCCGTGACGGCATTGCGGTACCAATGGCTTCGTATCTTTCCCACGCCATGGTGATGGGACAGTGATCCGCCGCAGGACAGTATCTCATCGCGGGCACTGTGCTCGATGGCCTCGAAAAGCTCGACGGGATCGGCCACGTCCGTACTGCGGAATCCAAAGTAGAAGTAGATGCAGGCACCGGCATCGTAGGTTTGGGTTACTCTACACGAAATGGTATAGTAGTTAATACTACGTTTGCTGCACTCCTGCAATGGTCGAGATGATAATTATTGTTTATATGAATAATTTTTAATATAGCACACACCGAAACAACACGCTGCTTCACAGAACGACAGAGCAGACTGCAGCGATCCCAAGGCACCGATGTCTCAAAGGACTCCGCCACAATTCCCTGATGTAGTCCAAAGTCCTTAAAAAATATATGAACATTAAATGAATATTTATATAAAAGATAATATAAAAAAGGTTTGTATTAGTGATAAATAAATCTTTATAAAAAGTTTGCCATTTATTCTTATATTAGTTACAGATCAATTGTGTTAATTATTTCCATATTAGCGCTGATATAATATATATAGTCCGCAATCGATCGAAATGAGCAAACTACAAATTAAAATCATTCTACCAAACGACTCGAAAACCAAACAACAATAATAAATAAAAATAGGGAAACGTAAAGCCGGA AAACCGGAAATAGCACATTGTCATAAAGATAATGATGTTGATCTTTACTTTTAGTTTCATTTGTACAAATTCAGAAAATACTCACCCTAATGTAGGCAATCACAAAGGTGAGTATATAGCCCCGTTCCCCATTTTGTCCACCTGCCGGAAATCCCTGAAACTTTTCGGCAATCTCGTAAATGAGTGCCTCCTGCCGCTGGACATCCTTTAAATCGCCCTCGAAAAGCAAGGTGGCCGCGCAGATCTGATTGAGATCGATGCCCTTCCAGGAGGTAACGTAGCGCTGCTTCATGGCATCCACCACACTGGCCCACCAGGACTTCTCCGGTTTCAGGGCCTGGCCGAACATGAACTGCTCGTTGTCCATCAGCCGGACGGAGGCGGGTTGACATCTCCTCCGCGCCACTTCGCGCATGAAGAGCACTCCCTGCTCGAAGTTGGGAAAGGCCAGGGATCCGTAACGCCTCAACGATGGCAGGGGACGCACCTTGAGCACCACTTCGGTGATTACTCCCAGAGTGCCCTCGGATCCCAAGATGACATGGTTGAAATCAGGTCCACAACTCACGCGCGGCGCACTGCATTCACGTTCCAGCGTTCCCGACGGAGTGACCATCCTAACTCGCACCACCAGATCCTCTATGTTACCATAGACGTTCTTCTTCATGCCAGATGCACGAGTTGCCACCCAGCCGCCCAGGGTGCTGAACTCATACGAATCCGGTTCGTGGCCAACTGTCAGACCTTCACTTCGCAACACCCTCTCCAGATCCTGACCCACAATGCCGGACTCAAAGCATACGGTGAGATTCTCCCGGTTTAGCCACAATAGTCGATTCATCTGAGAGGTGTCCAGGGCGCAGATCATTCGGCTCTCGTTCTGCGGACAGGTGATGGCTCCCGATACACTCGTTCCACCACCAAAAGGCACCAGCATCACATTGTGCTTGTTGGCCAGCCGCACCAACTGGACTACCTCATCGTGGCAACGTGGCCACACCACCAAATCGGGTATCCGTCGAAACTTGTGGTGCCACAGGCTGTATATATCGTTGAGGGTCTGGCCATGGCACCGCACCAACCGATCGATTCCCTCCGCAGAGTAATCCA
 
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