CR00304

CRIMIC CR00304

Gene CG4887

CG4887

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:1174828..1174843	21F2	FBti0199560	79235

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	no band	TTACCACAGCCTGATCAACGACGAC	ATAGTAGCCGGATGTCTCATCATACTGG

Images

Construct Design Sequences

sgRNA sequence	ACTGTGTAGAGTGTGAATGAAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGAGAAGGGGCTCCTCAGA	ACACAGTCACCGGATTGCAT	GGCATCCTAGCGATCGGTTA	AGGGTTCTTGGGCAATGCTT

Amplicon 5'	Amplicon 3'
GAGAGAAGGGGCTCCTCAGATTACGACAGCGATGAAGGCCACATGCGCAGGAACAAATACCGATCCACCACTGAAGCCCTCAATATTATTATAATCTTCGGTCTTACCAAGGAAATGACCAGAGCAGATGTAAGCCAAGCAGAAGAGTGGCAAATGCAACTCCTTTAATTACTCATGAACTAACTTTTTTTTTAGATTATGAGCGAGCTGATAAAGGTGAACATGGAGCCAGCGTGCATTCGCATTATCCGGAAGCAGGGAACAGGTGGGTTACGAAATCACTGACTTTGTTCTTTCAGCGTCAGACGTAAGTAGGTCCAAAAAGTTGGAGCGGCTTCGCGCTAATGGAGGCGAAGTGTACAAGTACCAATCGAATTTAACCGGCCACATGAATCGAAAAGAATACAATAGTCCACAAAAATGTTACAAACCTATCGAAACATTCAACATTTTTTGACGAAAACATTTTGTATCTGCTTCTATCCGAAAATTTAGATTCATCACGCGGTATAGCGTTTGTCGAGTTTAACACCGTTGAGGAGGCAAAGCAATGGATGGATATTACACAGGTATCAGCGCAATCGACCAGATCCCCTTGAAACTTTGATCATCATCAGCAGCCAAGGTTGTTCGCAGGCAATGATAATAAGGAATTCCTCACTGTTTAGCTTGCTTTGTCCAACTAAAAGTACTTATATGTAGTTGCAAGGAAACTAAGCACGAGTATAGTATTGCTTCATTAGAATTGCCCAAGAAAGCCACTCGAGGAGATTGCCAGCGGCTAAAACTTCGCGTTACGAAACACAGATGCAATCCGGTGACTGTGT	GGCATCCTAGCGATCGGTTACTAATCCGCAACCTTTCCTTCTCCTATTTCCATGTCTGGCGCACCAGGGCGTTCTGAAGTTAAACGACGAGCGGGTGAGCATGCGGTACAGCCACAAGCGAATTCAGGACTGGAACTGCAACAAGGTAGGTGCACTGAGATGATCATATTCTTAATGGCGAGTAATGTGTTTCGCTTGTTCCAGTGTGGCGTCTGCAACTTCAAGTTTAGATTCTATTGCTTTGTGTGCAAAACATCCCGGGAAGACAGCGAAACCACCTTCTCCTCCGGTAGCGAGGGCGTGGACGAAGTCAGCACCATCCTCACCAAAAGTATACAAACCACATAGACTCTCTTGCCATCTCGTTTTGTAGCTTCGATCGCAATGAAGTCCAACAGACCACTGACCGCCACGATTTCTCTACCACTTCCCCAATCCACGCAGAGATCATGCTACGCAACCTGGACGCCCTGACCAACGAAGAGGCTGTGCTCACTGCCCTCCAACTTCACCTAAAGGATCTGAGCAAGACCGTGAGCAAGGTGCTAATTAGTCGGGATTCCTTGACGCAGGCATCGCGTGGAATTTGTTACCTTCACTTCGACACTCTTGTCGACTCGATGAATGTGCACAATGCCTTGACAGCGCTGGATCCTCCACTTACCTTGGACGACAGAGTTGGTATGTGGCCTTAGGTGCTTTTTATAACTTAAATAATAACCAATATTTCATCCAGTGGCCATTACCTATTGCATCGACTTGGAGGAGCAGCAAGCATTGCCCAAGAACCCT

Amplicon 5'

Amplicon 3'

GAGAGAAGGGGCTCCTCAGATTACGACAGCGATGAAGGCCACATGCGCAGGAACAAATACCGATCCACCACTGAAGCCCTCAATATTATTATAATCTTCGGTCTTACCAAGGAAATGACCAGAGCAGATGTAAGCCAAGCAGAAGAGTGGCAAATGCAACTCCTTTAATTACTCATGAACTAACTTTTTTTTTAGATTATGAGCGAGCTGATAAAGGTGAACATGGAGCCAGCGTGCATTCGCATTATCCGGAAGCAGGGAACAGGTGGGTTACGAAATCACTGACTTTGTTCTTTCAGCGTCAGACGTAAGTAGGTCCAAAAAGTTGGAGCGGCTTCGCGCTAATGGAGGCGAAGTGTACAAGTACCAATCGAATTTAACCGGCCACATGAATCGAAAAGAATACAATAGTCCACAAAAATGTTACAAACCTATCGAAACATTCAACATTTTTTGACGAAAACATTTTGTATCTGCTTCTATCCGAAAATTTAGATTCATCACGCGGTATAGCGTTTGTCGAGTTTAACACCGTTGAGGAGGCAAAGCAATGGATGGATATTACACAGGTATCAGCGCAATCGACCAGATCCCCTTGAAACTTTGATCATCATCAGCAGCCAAGGTTGTTCGCAGGCAATGATAATAAGGAATTCCTCACTGTTTAGCTTGCTTTGTCCAACTAAAAGTACTTATATGTAGTTGCAAGGAAACTAAGCACGAGTATAGTATTGCTTCATTAGAATTGCCCAAGAAAGCCACTCGAGGAGATTGCCAGCGGCTAAAACTTCGCGTTACGAAACACAGATGCAATCCGGTGACTGTGT

GGCATCCTAGCGATCGGTTACTAATCCGCAACCTTTCCTTCTCCTATTTCCATGTCTGGCGCACCAGGGCGTTCTGAAGTTAAACGACGAGCGGGTGAGCATGCGGTACAGCCACAAGCGAATTCAGGACTGGAACTGCAACAAGGTAGGTGCACTGAGATGATCATATTCTTAATGGCGAGTAATGTGTTTCGCTTGTTCCAGTGTGGCGTCTGCAACTTCAAGTTTAGATTCTATTGCTTTGTGTGCAAAACATCCCGGGAAGACAGCGAAACCACCTTCTCCTCCGGTAGCGAGGGCGTGGACGAAGTCAGCACCATCCTCACCAAAAGTATACAAACCACATAGACTCTCTTGCCATCTCGTTTTGTAGCTTCGATCGCAATGAAGTCCAACAGACCACTGACCGCCACGATTTCTCTACCACTTCCCCAATCCACGCAGAGATCATGCTACGCAACCTGGACGCCCTGACCAACGAAGAGGCTGTGCTCACTGCCCTCCAACTTCACCTAAAGGATCTGAGCAAGACCGTGAGCAAGGTGCTAATTAGTCGGGATTCCTTGACGCAGGCATCGCGTGGAATTTGTTACCTTCACTTCGACACTCTTGTCGACTCGATGAATGTGCACAATGCCTTGACAGCGCTGGATCCTCCACTTACCTTGGACGACAGAGTTGGTATGTGGCCTTAGGTGCTTTTTATAACTTAAATAATAACCAATATTTCATCCAGTGGCCATTACCTATTGCATCGACTTGGAGGAGCAGCAAGCATTGCCCAAGAACCCT

Search Again