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CRIMIC CR00305

Gene Etl1

CG5899

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:9912014..9912025	30E4	FBti0199348	78887

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCGTGAATAATGCTGGTCTGTTCG	GCTTCATGATACGCTTTGCTCTCTGG

Images

Construct Design Sequences

sgRNA sequence	GAACAGCTGTCGTTAGCCAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGCGAGCCATGGTACTTCT	GCGAACAGCTGTCGTTAGC	CGAACCGAATCGCCATGTTC	TCACGGATTGTCGGGTACAG

 

Amplicon 5'

Amplicon 3'

CTGCGAGCCATGGTACTTCTCCACCACCAGTTCGGGACACCAGCGGCTAATCTCTGCCTCCCAATTATCCAGCGTGGACGAGGGCACCACAATTAAATGTGCAGCCTGGCTAAGACCATTCTCCTTTAGATAGGCCAGAAATGCAATAACCTGAATTGTCTTGCCCAGACCCATTTCATCTGCCAAAATGCCATTCATTTCTTGTTTGTGCATTACGGTGAGCCAATTAAGGCCAATTATCTGATAATCAGCCAGCTGTAGACTGCAAGAGGAACGAAAATAGAAACACAAATGGGGACATGCATGCTGGTTAAGGCTCACCCGCTGCTAAGAAGCTTAGGTTGCTCCACAATGCCAGCACCATTGGAAATCGCCTTCTCCAGACGCGACACCATGTTATTGCACTTACTTAGAATAGCGGCCACCGTGTTCTGTTTGTTAATCAGCTCTTGAGCGTAATTCAAAAGATCACCCGACATCCTGATGCTCTCCAGCTTTTGGCGCAAATCAGACCAATCACTAAATGGTCGCACATCAATGATGGCTAATGCCTTCTTCTCGGACAGCGTTTTAACTGACTGGAGTTCAATTAGCGAAGCCTCGTTCATAAACTGGAAAACTTTTTTACGTTGCCCCGTCATCTTGGTGGACATCTCGCTATCGCTGTCATCGCTATCATACACCTGGTCCTTGGACTGCTTCACATCATCGTCGTCCGAATGGTCGTTATCCGAGAAGTTGCCTCCACTAATTCCGTTTGACCTGGGTTTCAGCTTGGACTTGGCCAGTGGTCCATTGTGCCCCTTGGGCTTGCAGTTCTCCCTTAGGTATCGCACGGAAGCGGCCACATCCCAATTGGTGCGGGAAAGAGACTCCTGGATGGCCTACGGAACATATTTGAAATTTATTTCAGTTAAAATCGAGATTTATGCAAAATATTGCATTAAAAATGAGCAATTTTAAGCTATAAAATATTCTGATTAATTTTAAAATTCAAATAAGAACGGTGATAATGTCCGGAAATATTTTATATGAGGCACACCAAAGTAGTGGAAACAGTTACACGAGAGGGTTATTACTGCTCCCATCCCCGTCAGTGACGCCCCTGGCTAACGACAGCTGTTCGC

CGAACCGAATCGCCATGTTCCCCGAACTCAGATGCAGAGACGCGCGTTTCCCCGCGAATCGCCCCGTTTTACCTTGGTGGCATTTCGGCCGGAAACCTACCATTGTGTCAAAGTGGGGCGAAATCTTGGCGGCTGCCATGTAGCGCTCCTCCTTTTCCTTAACGGTCAGCTCCAGTTTGGTCTTCTTGGGCGTCTGGGAGTCGTTGCCATCGCTGTCACTATCGGCCATCACCTGGATGCGCTTCTTTCCAGGCACACGCTCTGAAATCAGGCAGTTAGCTACCGTGTCAATCGCTTGGAATTTTAGCTCAGCAAGTACCAGTTCTCGAAGGACTAGCGACGACAGAGGAAGCATTCTTGTTGATGCGGAACTGACGCAGATCGCTGAGGCTCGACTTCGCGGACGATGAGGCGGAGGCCGACGCAGATGCGGCCACTGTGCTGTCCGACATCGTGGGACAAGGCGAAACGCTTCCTGGCAATCTGTCAGTCCTTATTAACAAATTCTGTTTGCAACTCCTACAATCTTAAGTTGGTATTTGTGAATATTCGCATGCTAGTGCGGCCGTTTGATTATCCCGATAAAACCTATTTCTATTTCTCCAACGGAATGCGGTCACACTGTGCGCCAATTTAAGTATTAGCTTAATGATTATTATTATTTTTCATTTATTTTTATCGAACTGTAACGCATTAGTGTAAGCTTCTATGAGATTATCTAACAATTTGCTTTTGTATATATCGTATATGATTTGATTTCGTCAACCTAACCGTAAGTAATTAAAACCGATGCCAAAATGAAAGAAAGAAACTTAAAAAAATCCCAAACTGGCGGTAAATTAAGAAAGTTGCCAGAGTTGCCAGATGTTATCGGCTTACCGCAAGCAATCGGCCAACAATCGAAACATTGGGACGCGTTGCAACCCTCTCTTTTTTTCGCTTGCTCTTGTAGGTTAATTGAAAACACTTTTATTGCAAATAAACCGCTTGCCGAGCCAAGGGCAAACCGATAAACTGGCAAACATGGCTGGCGGCAACACCCCGCGTGTGATCCAGGTGACCAACATAGCGCCGCAGGCCACCAAGGACCAGATGCAGACGCTGTTCGGGAACATTGGCAAGATAGAGGAGATCCGCCTGTACCCGACAATCCGTGA

 

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