CR00305

CRIMIC CR00305

Gene Etl1

CG5899

Information

Donor Location Cytology FBti Stock number
pM37-p02L:9912014..991202530E4FBti019934878887

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GGCGTGAATAATGCTGGTCTGTTCG GCTTCATGATACGCTTTGCTCTCTGG

Images

Construct Design Sequences

sgRNA sequence GAACAGCTGTCGTTAGCCAGGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CTGCGAGCCATGGTACTTCT GCGAACAGCTGTCGTTAGC CGAACCGAATCGCCATGTTC TCACGGATTGTCGGGTACAG
 
Amplicon 5' Amplicon 3'
CTGCGAGCCATGGTACTTCTCCACCACCAGTTCGGGACACCAGCGGCTAATCTCTGCCTCCCAATTATCCAGCGTGGACGAGGGCACCACAATTAAATGTGCAGCCTGGCTAAGACCATTCTCCTTTAGATAGGCCAGAAATGCAATAACCTGAATTGTCTTGCCCAGACCCATTTCATCTGCCAAAATGCCATTCATTTCTTGTTTGTGCATTACGGTGAGCCAATTAAGGCCAATTATCTGATAATCAGCCAGCTGTAGACTGCAAGAGGAACGAAAATAGAAACACAAATGGGGACATGCATGCTGGTTAAGGCTCACCCGCTGCTAAGAAGCTTAGGTTGCTCCACAATGCCAGCACCATTGGAAATCGCCTTCTCCAGACGCGACACCATGTTATTGCACTTACTTAGAATAGCGGCCACCGTGTTCTGTTTGTTAATCAGCTCTTGAGCGTAATTCAAAAGATCACCCGACATCCTGATGCTCTCCAGCTTTTGGCGCAAATCAGACCAATCACTAAATGGTCGCACATCAATGATGGCTAATGCCTTCTTCTCGGACAGCGTTTTAACTGACTGGAGTTCAATTAGCGAAGCCTCGTTCATAAACTGGAAAACTTTTTTACGTTGCCCCGTCATCTTGGTGGACATCTCGCTATCGCTGTCATCGCTATCATACACCTGGTCCTTGGACTGCTTCACATCATCGTCGTCCGAATGGTCGTTATCCGAGAAGTTGCCTCCACTAATTCCGTTTGACCTGGGTTTCAGCTTGGACTTGGCCAGTGGTCCATTGTGCCCCTTGGGCTTGCAGTTCTCCCTTAGGTATCGCACGGAAGCGGCCACATCCCAATTGGTGCGGGAAAGAGACTCCTGGATGGCCTACGGAACATATTTGAAATTTATTTCAGTTAAAATCGAGATTTATGCAAAATATTGCATTAAAAATGAGCAATTTTAAGCTATAAAATATTCTGATTAATTTTAAAATTCAAATAAGAACGGTGATAATGTCCGGAAATATTTTATATGAGGCACACCAAAGTAGTGGAAACAGTTACACGAGAGGGTTATTACTGCTCCCATCCCCGTCAGTGACGCCCCTGGCTAACGACAGCTGTTCGC CGAACCGAATCGCCATGTTCCCCGAACTCAGATGCAGAGACGCGCGTTTCCCCGCGAATCGCCCCGTTTTACCTTGGTGGCATTTCGGCCGGAAACCTACCATTGTGTCAAAGTGGGGCGAAATCTTGGCGGCTGCCATGTAGCGCTCCTCCTTTTCCTTAACGGTCAGCTCCAGTTTGGTCTTCTTGGGCGTCTGGGAGTCGTTGCCATCGCTGTCACTATCGGCCATCACCTGGATGCGCTTCTTTCCAGGCACACGCTCTGAAATCAGGCAGTTAGCTACCGTGTCAATCGCTTGGAATTTTAGCTCAGCAAGTACCAGTTCTCGAAGGACTAGCGACGACAGAGGAAGCATTCTTGTTGATGCGGAACTGACGCAGATCGCTGAGGCTCGACTTCGCGGACGATGAGGCGGAGGCCGACGCAGATGCGGCCACTGTGCTGTCCGACATCGTGGGACAAGGCGAAACGCTTCCTGGCAATCTGTCAGTCCTTATTAACAAATTCTGTTTGCAACTCCTACAATCTTAAGTTGGTATTTGTGAATATTCGCATGCTAGTGCGGCCGTTTGATTATCCCGATAAAACCTATTTCTATTTCTCCAACGGAATGCGGTCACACTGTGCGCCAATTTAAGTATTAGCTTAATGATTATTATTATTTTTCATTTATTTTTATCGAACTGTAACGCATTAGTGTAAGCTTCTATGAGATTATCTAACAATTTGCTTTTGTATATATCGTATATGATTTGATTTCGTCAACCTAACCGTAAGTAATTAAAACCGATGCCAAAATGAAAGAAAGAAACTTAAAAAAATCCCAAACTGGCGGTAAATTAAGAAAGTTGCCAGAGTTGCCAGATGTTATCGGCTTACCGCAAGCAATCGGCCAACAATCGAAACATTGGGACGCGTTGCAACCCTCTCTTTTTTTCGCTTGCTCTTGTAGGTTAATTGAAAACACTTTTATTGCAAATAAACCGCTTGCCGAGCCAAGGGCAAACCGATAAACTGGCAAACATGGCTGGCGGCAACACCCCGCGTGTGATCCAGGTGACCAACATAGCGCCGCAGGCCACCAAGGACCAGATGCAGACGCTGTTCGGGAACATTGGCAAGATAGAGGAGATCCGCCTGTACCCGACAATCCGTGA
 

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