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CRIMIC CR00311

Gene Vinc

CG3299

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:2219287..2219297	2E3	FBti0199562	79237

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CCATCGAGAGCATTCTAGATCCTGTTGC	ACCCTCTTGCACTCCTGGATGATCTT

Images

Construct Design Sequences

sgRNA sequence	TATAGGCTACATGCTTTAGGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGAATCGGCCTCCCTTGAAT	AGGCTACATGCTTTAGGAGGT	AGCCTTTGATTTTGCTCTGAC	GCTTTCCCCACCGTAACTCA

 

Amplicon 5'

Amplicon 3'

GGAATCGGCCTCCCTTGAATATTAGCATAATCTACCCTTTGCAGGAATTCCCCAGCTGGTATTATTGCGATAAAATAATTCGTGTTTGAATTGGCAATCTTGTTTCACATTCGATTTGTCGATTGGGGGATTTTGAATTAGAGACAAAACAATTGGATATCTGTTATATGAATGCGCCTCCATCGGTGACCAGTGATTACTTCATTCCCGTTCAAGCGCGATGATTGATCAATCAATTCCAATGGAGTTGTTTATGGCAGCAAAACTGTTAATGATCTTGCTGCGTGATCGCTCGGCGGCAGGCCCATTTGGACTTATCGTCGATCCAACGGCTTCGCGCTCCGTTCATTCATCGACTTGGGGAGCTGGTGGAGAGCGCTAGAAATGAAAACGGCGTGGGCGGCGCCATGGAACGCCACATGGTGCACGGAATTTGGACTTGACATCAGCACCTGCTCAAGGTGTTTTTGGCCATGGCCATCTGCCTATTTATAGGCAATGAGCAGTGCGCAGCGTCCGCCGACGCTAATCTCCACTGAGCCCCATTAAACCGAAACTAAATCGAAGCGCGGCTAAGTCAAAGACTTAAGACTTAACTGGTGTAAGCTGGCGGATTAGGTGGTTAGCCACTGAGTGCTGGGCAATACACGTCCGGGGTGCACCCTCCTGCTCCAGACCCCCCCCCCCCCCGCGTTGGGCGCCACTTTTGCAATTAGCAACAATTGGCCTACTTGATTAATTAATTATGCAATTAAGCTCCTAGTCGCTAGTTGGAAGGCCCCTCGCTGCTCATCCAACGACTAATTATACGGACAGAGACATGTGTGCCCGACTTATGTATCCATGCGCTGATGGATGTGGTGAATGATCGTTTTTAATTCATTTGTTTGCCAGCAGCACCCACCACACTCAATCCGCCCCACGGCGGATGACTAATGCACTGGGTTTGCTTTTTGGCACAGATTTCAGACCACAATCCTCACTCATCCGCGTCGCTACAGATGTGCTAGGTACACCTACAACTCCCACACCTTCGGTCCTCACCTCCTAAAGCATGTAGCCT

AGCCTTTGATTTTGCTCTGACTTTGCTTTCGCCATGATTTCATTTTCATTGCTTGTCCGTCGTAAATGTTTTTATTTTGGTTGTTTCCTCTCGCTGACGCCGAATGTTGTTGCCGCCGTTGTTGGATTTTACTAAACGTATTTTATGCTGCTATTTTTGGAACTCGGCCAAAAGGCACCCCCCATCCCCGATGCTCCGATCGCCACTTATTTGTTTTCTTTGCTCCGCCTGATTTTTCATTTTCATTTGTTGCGTTATTAAATTAAAATTCTGCCTGTCCTCAGAAGCTCAGGTTGGCGCTGGCCTCTGATCAACATTTATTCTTATTATTTCCACCTCATTTATTTTTAGACCAGTTAAGTAACGCCCGAAAACAAAACAACGAAGACGCTGATCGCTGATCGCAAATCACCTCCGCTCCGCTCGTTTGTTTCTTCGCCTCATGATGGAAACACACATGAATAGATGGATATATATGGAGGGGGCGATCGGATCGGAAAGGACAGAAAGAACTGTGGCATAAATCTATATATAATATATGGCAGAACACCCAACACATGCCACACGTATGCAGTTGGGGGAATCAGGTGCGAATGGCAACTATAGCTAACGATAGGTGATCGATCAGTGAGTTACGGTGGGGAAAGC

 

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