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CRIMIC CR00312

Gene CG15923

CG15923

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:20613235..20613239	92F3	FBti0199349	78888

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGTAATTTCTTCATCTGTCCAGCGAC	CCTCTAAGTTTCGCAGATGAATCAAACCC

Images

Construct Design Sequences

sgRNA sequence	CGGATTCGGCTTGATCGACTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTTTGTACGACGTGCTCGA	TTGCCCGTATAAGATCTGAGATC	GACGGATTCGGCTTGATCGA	CTCTGCTGGCGACTACTCTG

 

Amplicon 5'

Amplicon 3'

TGTTTGTACGACGTGCTCGATTAGCGGACGTTGATGTGTGCTAGCTATCCTATTGCAAAACACGAGAAAAGCCAAGTAGATCATGCGGGGCATCTGCTAATGTTTGTATGATAAATGTGAAACTATGGAAATTATTTTATCTGTTTTAGCCGTAAGTTTTACGATAACTAAAGATTAACCACTGTATATGAATGTACTACTTACTATCGAGTGGATTATGAAATAATAAAAAAAAACTTCCTGTAAGGGCTCGCATATCATTTATATGGCAAAGAATCCATCCTCTCGCAGATATTGCTCCTCGTTTTGAGGCTACAAGAGGAACCTCTCGTCGATGAGGGTCTTGCGGGAGATGTTCCTTCGCACCCAATGGCGGAACACTTGGCGTAGTAACTGAAATGGATTATTTTTTAATTATTTTGTTAATCAGTTTTGAATCATTATCCTACTATGTTTAGGGCACAAGCGCCAGCAGCTGCAATTAACAAATTAGAGGTCATCAAGAAGATCGACAGCACCAAGAGGAGTTCCAGGACAAAGATGTGCAAATGGTTACCCAAGAGGAGAACGCGAGAGAAGCTGAACGAGTCGTCTATGTAAAAGGTACCTCTGTCTGAAAATTTAAATGGATTTAATACATAAAAGAGAAACTTCGAATTGGGTACTCACTTTCCGTGATGTAGTTGTCTATTTCGCAGTTCAGCAATTGTTCCACAAACGACTTTTCCTTAATAATGTAATCCATGTCCTTGATAGCCTGTCACAAATATAAAAAGAGAACATTCCATCTTAGAATAAAACTGAGCTTACAAACAGCAAAAAGAGTTGAGATTTATTAGTTACTAGGTATTCTAGACTGTAGCTCTTAATAATGCTAGAATCTGTAAGCTTAGTCTTTCGATTTTTTAGATCCTCAGATCTCAGATCTTATACGGGCAA

GACGGATTCGGCTTGATCGACTAGGCCATATTAACTGGTTACATACTTCAATGGATCAATTAAGGTAGACTTTAAGCTTACATGGTCGATAAAGGCGCAGAAGAAGCGATTGACGCTGGCGTACGCAATGGAGGTACGTTCCATCTGTCCATCGATGCTCGGAACGATGTCCTTTTGGTAGAGCATGGTCTGCGAAAGGGTGCCGGTCAGACTGCGCTGAAAGGGAAGCAGGAGTCGCTCCAGGTAGATGCTATAGTAAAAACACTATGATAATCAATATCCTCATTGGAGTAAAGTCAAAATCTTACTGAAGGTTTCTGGGAGGCAGGATGACGTACGCCTGCTTGTCCGAGTCCATTAGCAATCCTCGTCGCCCACACATGGTCTGGGTTCTCTGCAGCTGCAAAATCATCGAGGACATATCCGTATCGGCAAAGCCGTGCGGAGATCGTCCGTGGATGTAGTAACCAAATCCTGGCTCCGTCAGTGAGAACAGAGAGATGTTGGCCAGACTGCACAGGTCGATGAACTTCTGGAGGGGATTGGCTATAAACAGTTGGTGGCCTATTATCTGAAGTAGATATGTGAGCAGATAAGCTGCCGCTATAAGGCAGCAACGTAGAAAAATGTCACCCTTGGTAAACCTCTCCAGAAACTGATAGCCAGCGATGACCACAAATACCTGACCCAAGGTGGAGTACTTCTGGGTGACACTTAGGCGAATCCAGGCATTGGCCGTGAAGAGAACTCGCCAGGCGGAGACGCTGCTCTCGGCCACAAAGGTGCGCACGCTAGAGCAGAGAGATGAGCTGTCCAGATTCAGGCGTTGTCCTTCGCAGCTGCTCCGCGGTCTTTCCCAGTCGATGAGAAACAGTTCCAGCTGGCTGGAGCGCCACAGGTGGATGCCCAGAAAGAGGAACTGCAGCACAAAGGCGGTATTGAGTAGGATTTTCAGTAGGCGAGAATTGGAGGCTTGCAGCAGTAGGAGCAGCAGAGTAGTCGCCAGCAGAG

 

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