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CRIMIC CR00313

Gene AIF

CG7263

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:2152490..2152499	22D1	FBti0200534	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	no band	TCTACTAAACGAGCGCGTGACCTATCCT	TGGAACACCTGGTACACTTTACCGC

Images

Construct Design Sequences

sgRNA sequence	TGCATTAGCTGGATAAGTTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGAACTCGCAAAACACACGT	GGTGAATGGTATGAACGATGGG	GCATTAGCTGGATAAGTTGGGG	CGCGTCATAAACCAAGCGAG

 

Amplicon 5'

Amplicon 3'

CGAACTCGCAAAACACACGTTTCTTTCGCCGCACAACGTTGTTACGTTACACAATTGTGGATTGTGGTGTCACCTACTCCGCAGCCAGTGCAAATCACGATGAGTATTTGGGGGGTCCGATGTCTGACGCAGAGGTTCATCCGGCAGGCGTACATCCTGGCCAACAGAAGGCTATTGGGTCCAGTGCCGCAGCGCTCGCCGCCGGCATACGGTAAGTGAGGTTATGGCCGGCTGGCCAAACCGATCCGTGTTTTCATGTTTTTGCCGATAACTAACTAGCATTCACGTCGATTTCAGCTCCTCTTCGGCCAGCCCACAGCAGCCTCTATCAAATGGTAAAGAAACGCACCCTTGAGGCGCGCACCAAATTGCAAGCCAACAAATATCCAAATCATCAAGTCTGCGTCACCAAGCCCTCGACCACGCCCCCCGTTGAAGAGTACGAGACGGCGGTGGAGGGAGCCGGCGTTCCCGCCTACGCCAATGCCCAGTTTCAAGCCCATTCCCAATCGGAGCCACTCTTTAAAGTGGTACCCATCGTTCATACCATTCACC

GCATTAGCTGGATAAGTTGGGGATGCAAACTAACTTCCTACAAGCCTTGTGAAATTGATAACTAGCGAAAACATAGCTGGGTTATTAAACAGAAACCAGTTTGCATCCCCATCAAAGACTTGCAGAGTTATTAACAAATATTAAACAATATAATCAATTTCCAGGGCTTTGCTGACGTCAAGTCCGTCTGCAGTGCTAAGGATGTGTTAAAGTCGGACAGTGCAAAGCTCAGCACCAGCCAACCCGTCCTGGATTCCTGCAAAGCGACTAGTCCCTGCGAGGAATTCAAGCGCAAACGAAAGGAGACCACCTGCCAGCCCTGCGACGAGGATGGTACTGCTCCCGGTGGCGGTGATGGTGGAGACGAGGAGTGCGAGTGTCGGATGAAGGACCTACGGCTTAAGTGCCTCTTGGGTGCCCTGGCTGCCTTGTTAGCCGGAGGATTTGTATGTTTCGATCTGATATCTAAAGGAAATATTCTCTAATTACTTTGCTATCATATAGCTCGCTTGGTTTATGACGCG

 

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