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CRIMIC CR00315

Gene HisRS

CG6335

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:18518554..18518557	17C1	FBti0200535	83168

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CACACACACACACAAAACACCGACACAC	TAAGAGTGCTCCGCCTGCGAGGATTTT

Images

Construct Design Sequences

sgRNA sequence	TAGCAAATTGTAGGCTTCTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGATCGATGAGGAGGTCGC	ATCACTTAACTTGGGCGCAG	AGCCACAGAAGCCTACAATTTG	GCTCTCCGTTCTGCTGTGAT

 

Amplicon 5'

Amplicon 3'

CAGATCGATGAGGAGGTCGCCCGCCTACTGGCCCTAAAGGCCACTCTTGGCGGAGATGCCGCCCCCACCAATCAGAAGTTCACGCTAAAGACGCCCAAAGGCACACGGGACTATGGTCCTCAGCAGATGACCCTGCGCCAGGGCGTCCTGGACAAGATCGTCCAGGTGTTCAAGCGCCACGGCGGCGAAGCCATCGATACGCCCGTGTTCGAGCTGAAGGTGAGTTCACCTGGGTCACATTGGAGTGGGATGATATTGTTCAAGACTATGAGAATTGTTGGAGGATTATGTAAGGAGTTTTTGTTTAGGTGAACACCTAGAAAGGTATCCTGAGGTGTCTAATAGCTTAGATTCTATTTCATTATACAATTTAATGATCATAGAAGAGCTCTATACTAATGGATTCACTAACTTAAATTTGAAATGTCTAACTAATTAGGCTTTATTATGGGTCTATTATTAACTTAAACTCTACAATTCATGAAATGTCCAATTTAAAAGAGGGATATTATAGATTGAAGTGGTTTCCAAAAACAGGTACAAGGTAAAAATGCAGGATATGGTGTCATCAATTCCCAATTAGTTTCTGGTAGCTTATTCGGTACTTTAATATTATAATCTCAGAATCAACAAAAATGACCTTGCTTCATTTTGTTATTAACAATAAATTATTTATATTGATAGGGTCTTGAATGATTTATATATATATAATTGTGATAGCACTGAGCTGCGCCCAAGTTAAGTGAT

AGCCACAGAAGCCTACAATTTGCTAGCACTTAGTATACACAGCTTTGTTTCTAAATCTCTAGTATTCAATTGGAAACATATACTATAAAGACTAAAGTAACATAATTTTAATGGCTCGCCATCATTAATTTTGGTATAAAAGTAGTTAATTAGGTATTTAAGCAGAAACCACAAGTAAAAATTGTATGGTATTGTGGTATAAATCCTTACACATTTCATGTCATGTGTCTAGATATTCGACTTATTTTTCTATTATCCATCACCACAGGAAGTGCTGACGGGCAAGTACGGCGAGGACTCAAAGCTGATCTACGACTTGAAGGATCAGGGCGGTGAGATCCTATCGATGAGGTATGACTTGACCGTGCCGCTGGCTCGTTACCTGGCGATGAACAAGATCTCGAGCATCAAGCGCTACCACATCGCCAAGGTCTATCGACGCGACAATCCGGCAATGACCAAGGGTCGCTATCGGGAGTTCTACCAATGTGACTTCGACATCGCCGGCACCTACGATCCCATGCTGCCGGATGCCGAGTGCGTCAAGATTGTGTCGGAGATACTGGACACGCTGGACATTGGAGACTATGTGATAAAGCTAAATCACCGCCAGCTTTTGGACGGCATGTTCCAGGCGTGCGGAGTGCCGGCGGATAGCTTCCGCACCATCTGCTCAGCGGTGGATAAGTTGGATAAGGTGAGCACAAACGCTGCTAACTGCAATTGTGGTCAATATTGTAATGTATCGGTTATTCCAGTCGCCCTGGGCGGATGTGCGCAAGGAGATGGTGGATGAGAAGGGTCTGGACGAGGCAGCAGCGGATCGAATTGGCGAGTACGTGCGACTGAGCGGCGGTGCCGAGCTGGTGGAACAACTGCTGGCCAACGAGAAGCTCAAGGCTGTGCCAAATGCCGTAAAGGGTCTGGAGGGCATGAAGCAGCTCCTTAAGTACTGTTCCATATTCGGTCTGGATAAGCGAGTTAGCTTCGATTTGAGTCTGGCCCGTGGCCTGGACTACTACACCGGTGTTATCTACGAAGGTGTGCTCAAGGGAGAGTCCGCCACCGTGGCATCTCCGGCGAAGACATCACAGCAGAACGGAGAGC

 

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