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CRIMIC CR00316

Gene CG1673

CG1673

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:13399167..13399167	11F1	FBti0199563	79238

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	short band	GCTAGCAACAGGCCGAAATCGGAA	AGGTTCTACTGACGGGTTCAGGTTCA

Images

Construct Design Sequences

sgRNA sequence	CGACTTGTTTACAGAGATTGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTCAGAGATGCAAGGTGCC	CTGGGGACTTGGGGATTACG	CCCAGACCGCAATCTCTGTA	CGAGGGGGAAATGCTCTGTT

 

Amplicon 5'

Amplicon 3'

ACTCAGAGATGCAAGGTGCCATTAACTACACTTCTTTACAATTCGGGAATTGGGATTAAATAGGGTCAAGGTTCTTGCTCTTCAAGAGCTGTCAGCAGAGCATGATCAAATCGACTTATGTGTTCAATTTTTTCACACTTGCAGGATATTTTCCGCAATCAGCATCGCCTGATCCGCTTCCTCGAGCAGTCGATCCGGTTGTGCAGCAACCAGTCTCTGAAAGCCGCCCAAGCGGCACAGTTGCAACAGAAATCGTCGGCGGACTTCGAGATCACCGCCTCGCTGGACGCCCAATACACCAGTGCCCCGGAGCCCATTAAGCATGACAAGAATCTGGGTCATCAGTTCCGTGCCTCACAGATTTCCGTGCGACTGGCCGCACCGGAGCAACTGCAGCCCAAGCCGGACAACGATGAGGAATTGGGTTTCGGCCGCCTATTCACCGATCACATGCTCAAGATCTACTACCACAAGAGTTTGGGCGGATGGCAGCGACCGGAGATTACGCCGCTGGAGAATCTAGTGATGCATCCCGCCGCCAAGGTCCTGCACTACGCCGTTGAGGTAAGCCATCGGTCCTGCGTTTTCACACTAAGAACATATCAGTAACAGCAACAAAAAGCATAGCTTAGAAAAAAGTTGAACAATGTAACTAGAATTCCTTGTTGAGTGCAAATATTTACGTGACTTGAAAACGCTTCGTAATCCCCAAGTCCCCAG

CCCAGACCGCAATCTCTGTAAACAAGTCGCACGTGTGACTTGTGAAGCAACGTCATGCAGATTTATAGTATATATGTACATATAGTGTAGTCAGAACCCAAATCGAAATCAGCGACTGTTGCCAATGGTGCGATGTTGTCTTATCACCTCTGTAATGCCATCCATTCCATTGCAGCTCTTTGAGGGCATGAAGGCGTACCGCGGCGTCGATGGCAAGATCCGCATCTTCCGGCCGGACATGAACATGAACCGCATGAACCTGGCCGCCCAGCGATCCGGTCTGCCCACCTTCGAGGGCAAGGAGTTCGTCCAGTGCCTGTCCCGCCTGCTGTCCATTGATTCCGAGTGGGTTCCTCACACGGACACCGCCAGCTTGTACATCCGCCCCACACTGATCGGCATTGATGTGAGTATATCTAATTAACCGTATCTACTTTGGAATATCATGAGCTTACCTGTTTTGATTTCGACTTGCAGCCCACACTGGGAGTTGCTTCCTCCGACTCGGCCCTGCTCTACACGATCCTCAGCCCCGTGGGCAGTTACTTTAAGACAGGCTCGTCCGGTGCCGTCTCCCTGCTGGCCGATCCCAGTTATGTTCGTGCCTGGCCAGGCGGCGTGGGCAATCGCAAAATGGGCTCCAACTACGCACCCACAATCAATGTGCAGAAGGAGGCGGCCGCCAAGGGACTGCAGCAGGTGCTGTGGCTCTACGGCGAGGATCATCAGCTAACCGAAGTGGGCACCATGAACATCTTTATGTTCTTCGTGAACGATCAAGGAGGTTGGTGTCTAATACAGCAGCAAAACGAATGGGATTGCTTATATATATATATCTATGTCATTTGCAGAACAAGAACTGGTTACCCCACCGCTGAGCGGTCTAATCCTGCCCGGTATCACCCGCGACTCCATCCTGCGCATGACCCGCCAGTGGGGCAAGTTTAAGGTTAGCGAGGCGAACATCACCATGCCCATGGTCTGTGAGCTCCTCAACCAGGGAAGGGTAAGTAATCTAACAGAGCATTTCCCCCTCG

 

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