CR00320

CRIMIC CR00320

Gene Rnf146

CG8786

Information

Donor Location Cytology FBti Stock number
pM37-p03L:19641599..1964161676B11FBti020053683169

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short band AAAGTCAGCCCGAGCTGGTCATACTCA GAAATGGATGTGCGGAATGGGTAAGGAG

Images

Construct Design Sequences

sgRNA sequence TGGTAGTCTTAGGCTAAAACTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTGGTCCGAGAATCGTACGC ACCACTCCAATTAGCACTCG TGGTTGAAATTGGCAGGAGA TCCTAGAGCGCATACACACG
 
Amplicon 5' Amplicon 3'
TTGGTCCGAGAATCGTACGCCCACCAGCCCCCTAGATGGGTTTAGTGCGTGAAGGATTTGGGGTTTATGGGAATGAGGGACGGCCGGCACCAAAAGCTTAATTATACTCACCGTTTCTTCCCTCGTAATACCACTGGAAGCCGTCCTCGGTCGCGCGAGTGGTGCAGATGTCCTCAATGCCATTCACCAGCTGGGGATGGTCCAGGAACTCGGCGGGAATCTCGCGACGGCACATAGCACAACGGCGGTTCTTATAAGCAACACCCTGGAAAGCGTAAAATGACCAATGAGAAATTAAAATTTGATTCGACATCGCTATGTGTTGAAAAGCAATTGTAATTTCCTAACACATAAAACTTAACAAGAAGCGAAAATAATACATTTCAGAAAAGCATCTTATCATTAGCGTAAAAACGCAAAAATACGATCATACCAACCTAAGACCTTTACCCCACATTAAGTACACTGGAATAAAATATGAGGTCTTTTACCTCAACCAAAAAATAACAACTTGTGTTAAACGTTATAAGCATAGATGAAGTTTACAAAACTAAGGAAGTTGGAATCGCCTCTCTAAACCAACACTTTCCCACGACCAAACATGTTTCTGAACAGGAAAATGCACTCGAGTGCTAATTGGAGTGGT TGGTTGAAATTGGCAGGAGATTGAGACCAATTGACAAGTCGTCAGCCGGAAGCCAATCTAATGCCATAATTTCTGGTCAGCCGTGTTCGGTTGGCATGCTAATCAATCAGTGAAACCAGCTGTCGCACCAAAGGGTTAAGAACAGCCTTGAACTTGGCTACGTGCTGTGGGTGATTTTGCTGATCGGGAATAATATTCAAGAAAAGCGAGTTTGAATGTGAATTGAATGACTAAGTGGCCACGAAAGGCTTCCGCTTTCGGCTTTCTGGGCTACCTACCTTCACGCACAAAAAGCAGAAGATGTGGCCACAGGGGAGACGAGCTGGGTGGATGCAGGTTTGCAAACAGATGGGACACTCCAAGGCAGCGGCCGCTGCAGAGGGGGAATCTTCGCCAGAAACGGCTGCCGCTAGAAATTGAGTTAATTGAAATAAGTTAAATCAAATAGAATAAGGATTAAGGGATATATGAATAATAACGATGCAAAAGTATAACTCTTGTGCTCTCAATAATATTATAGAATATTATTGTGCTAGTGAGTGCAAAATAATCTGTCATAATTCATAATTCCTTCTAGTATTGCTTTGAAATGCTTTTAGGAAGTCCTTCAAATTTGATTTCGTACATATGTATATGTACATATGCGTGTGTATGCGCTCTAGGA
 
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