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CRIMIC CR00322

Gene BRWD3

CG31132

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:24319793..24319806	95F12	FBti0200537	83170

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	GCTCCGATATTCATAACTAGTGGTGTGCG	ATTGCCAGAAGGCGATCGATCCATCTGT

Images

Construct Design Sequences

sgRNA sequence	AGACAAGCGGACGTACAAGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCTCCTTCGGCTAAGACTC	GCGGACGTACAAGTGGGTT	TGGGAATCAGACTGAATTGTGGA	CCGTCCAGTTAGAACGCGAT

 

Amplicon 5'

Amplicon 3'

GGCTCCTTCGGCTAAGACTCGATACCCATCTTGTGAGCCCCGCCACCAACTATCGATACGCAAAGGCATTTCATTCGGTGGGAATGGCAATCGAGTGTCACACGGGAAGAAGAAGCTGCGAAAAGAATTTTAGAAGAAAGCAACTGCAATTTTGTCATAATCGGAGCTGCAATTCGCAATGAATAATGGAAACTAGACAACCCAGTTCCGGTGATGATCTAAAGCCAGGTGCGTACCGCCCGGTGGCGCAGCGAGTGCGTCCAACGGGGCTACCAAAGGCAGCCCCTTTGACTTTGTGAAATGTGTGTCTGTGTTTCGCTCGCCCTTCGAATCGAATGGCTGCGTTTTTTCGACTGCGTTTCGCCAAGCGAAATTCACGGGCCCCTTTGGCCAGATATATCTGGAATATTGACGACTCCCAGCGAGCGCATTTTCGACAACTTGTTGCACTAAAACCCGTGAAATCCTCTATCTTGCAGAGCTGTACTTCCTCATTTCCAAGTTCCTGGCCGCGGGACCGCTGGAGGAGACGGCGAAGGTGGGTTGAAAGCAGCGCTCGCGCGAGATCGCATTGGAATCGTATTTCGCGCTAAAAGAGCGGAATTTCGTTTTGTTTACAAACATTTACATTAATTCTGAAACTAAATTCTATCCAACTGTAGGTCTTGATCCGGGAACTCGAAGAGAAGAAGGTGAGTTTTGTTGGTGCTGCAGCTGTGTTTTTGGCACAGATATTTCGGACTCGAATTGAGAGGGGGGCCAAAAAAAAAATAGAAAACCGCGTTTGTTTATACGCTTTGTTTTGGTTTTGTTGTTGTTTCTGCTGCCGGCAGCGAACGACGAAAAACCCACTTGTACGTCCGC

TGGGAATCAGACTGAATTGTGGATTTGCTGGGGCCACGTGAATATATAGTCCATTAAAAAAAGAAGAGAAAAACATGAAAAAAGAAATGGAATCATAGGGATGCCCCCGCGACGTCATCGTCGACGTCACGGAGTGCAAGTGATAAGAGTCATACATGCATGCATATATATTTACACCCGTCTATATATACGTACACCTTTCGATTCCCAGGTTTTGCCGCGTCGTCTCGATTGGCAGGGAAACGAGCATGAGCAGACCTTTGAGGAATTGGTGAGTGTTTTCATAATTAATCAATTAGCTTTTCAAAGTTATCGTTTAATGCCTTTTGATCGGCATTTAAGTCTTGCGTAAGTGTGCGTCTGCTGCGGCATTTTCCGTCTCAGTCGTTCGCTCGCCTTTGTGACGTCATCGTGTCAGGGAATAGAGAAAAAGTTGCACATCAAAATTCAAGTTGTTAATTTAAAAAACATTACAACTAAAGACATCAATCATTCTGACTGTTAAATTAACAAATAAGTAAGCAACTATTTAATTAAAAAGGCAGCAAAAAATTGCAAAAATTCGTAAAAGACTAAAGTGGAAAGCAAAAATTCTAAAACCCTCGTAAAAATTGTAAATTTTGAAAAATCTTAATTTTTTAAACAAATGCTTTGTAATTTAGTTTTAACAATTAGCTGCAACCAGCCGACATTTTGGTTTCTCACACATACACATGCCCATGCGCACAGACACATGCAAGCAACCACCACTTTGCCTTGACTTGTGCGCGAGGGAACGAGACAAGCATACGTTGTTGAAGCTATCGCACCGTTTATCGCCCAATCGATAGGAATACGTTAAATTTTGATTTTGAATTCGCAGGAACGAAAGTACAAGCACATTGGCGCCAATCACTTGCTTGAAATATGCAGTCGTCTGGGTCCACTGGTGGATCGCGAGCTTCCGCCCAGTGTTCCGGGCATCAACTCGCTGTTGGGTACTGGGCGCCAGAACCTCCTGCGCACCAAGGAAACCGTCTACTGCCATCGCTCCTTAAGGGACTACTGCACCCGCCTGAATGGAGTCTCGCTGCCGGATTCGGTCCTTACAAAGCCGACCCACAACTTAGGTTAGTTGCATGGTAGAGTTTTATTCGCATATTAGAGACTAACCCATTTATATTTCCAAAGATCGCGTTCTAACTGGACGG

 

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