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CRIMIC CR00323

Gene Marf

CG3869

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:6368441..6368441	5F4	FBti0199350	78889

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGCGCCAAAAAGAAGATCAACGATATC	ATACTCACTGCTGCACTTGGTGGCT

Images

Construct Design Sequences

sgRNA sequence	TGCTTTGGGAAGAGTCAACAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CATTCGCGAAGTACTGCAGC	GGTGTTCATCGGTTCAATTCGA	CCTCCTTGTTGACTCTTCCCAA	ATACGCTGTGCTGTGAACGA

 

Amplicon 5'

Amplicon 3'

CATTCGCGAAGTACTGCAGCGGGATCACATGAAGGTGGCCTTCTTTGGACGCACCTCCAACGGCAAGAGCTCGGTGATTAATGCCATGTTGCGCGAGAAGATCCTGCCCAGCGGCATTGGGCACACCACGAATTGCTTCTGCCAAGTGGAGGGCAGCAATGGCGGCGAGGCGTATCTTATGACAGAGGGCTCCGAGGAGAAGCTAAATGTGGTGGTAAGTGGGCTACTCAGCTCTCCTCTGGGAAATGTGTTTCGCCTAATGACCTAGTGACCCTTGTTTGCAGAACATCAAACAACTGGCGAATGCCTTGTGCCAGGAGAAGCTCTGCGAGAGCAGTTTGGTGCGCATCTTTTGGCCGAGGGAACGCTGCAGCCTGCTGCGCGACGATGTCGTCTTTGTGGACTCACCTGGTGTGGATGTGTCGGCCAATTTGGACGACTGGATCGATAACCATTGCCTGAACGCCGATGTCTTTGTGCTGGTCCTGAATGCCGAGTCAACGATGACGCGTGCGGAGAAGCAGTTCTTTCACACCGTCTCGCAGAAGCTAAGCAAGCCGAACATCTTCATCCTGAACAATCGCTGGGATGCGTCGGCCAACGAGCCCGAGTGCCAGGAATCGGTATGATCTTCTAGGCGGCCCCTTTAGTTAGTCCACAATCTCTGTCAGACCCTGCATTACCATTACATGGCAGGCGAACCATATGCCACATATGTGTACATATGTTCGTTTGCCAGCAGTTTTGCACTGCCACATCTCATAAGTAATTTCTTGGACCTCTTCGTATGCCTGTTGTCTCTCTATGTTGATGTGTGTCTTTTGTTTTTGTTTATTACCCAACCAACCAATCAATCTATCAAACAACCAACCAACTATCCACTGTAATCAACGCCAGGAATTGGCTAAGGTAATGAATGAGCTAGACCCGCTTGCTTGGTCCCACAAAAACCCAATCCACTCTGCTTTGGTCTCTTTATCGAATTGAACCGATGAACACC

CCTCCTTGTTGACTCTTCCCAAAGCAGCTTACTTAGTTGCCCTGATTGATTGATCTATGCACTTTCTAAGACGCTTATTTCATCAATTGATAGGTTAAGTCTCAGCACACGGAACGCTGCATCGACTTCCTCACCAAGGAGCTAAAGGTGAGCAACGAGAAGGAGGCGGCCGAAAGGGTATTCTTCGTTTCCGCCAGGGAAACGCTGCAGGCGCGCATCGAGGAGGCCAAGGGCAATCCGCCGCACATGGGTGCCATTGCCGAGGGCTTTCAGATACGCTACTTCGAGTTCCAGGACTTCGAGCGCAAGTTCGAGGAGTGCATCTCGCAGAGTGCTGTGAAAACAAAGTTCCAGCAGCACAGTTCGCGCGGCAAGAGTGTTTCGGGTGACATGAAATCAATGTTGGACAACATTTACGAGCGGATCACCATATTCCGCAATCTGAAGCAGGACCAGAAGAACCTGCTCACCGAACGCATCCAGGGCACAGAGACGCAGATGATGCAGGTTACGCGGGAAATGAAGATGAAGATCCACAACATGGTCGAGGAGGTGGAGGAGAAGGTGTCAAAGGCGCTGAACGAGGAGATCTGGCGCCTGGGCGTACTCATTGACGAGTTCAATATGCCCTTCCATCCGGAGCGTTTGGTGCTGAACATCTACAAAAAGGAATTGAATGCCCATGTGGAGAGCGGCCTGGGCAGCAATCTGCGCGCCCGCCTATCCATGGCCCTGGCCATGAACGTCGAGTCCGCCCAGACGGAGATGACCGATCGCATGCACGCCCTGGTGCCCAACGAGCAGCTCCTGGCCACCAGCACCAAGATGGTGGTGCGCACGCAGCCCTTCGAGATGCTCTACTCACTGAATTGCCAGAACCTCTGCGCCGACTTCCAGGAGGATCTGGAGTTTAAGTTCAGCTGGGGCATTGCGGCGATGATCCAGCGCTTCACCGGCAAGGTGCGCGAGCGCAGCAAGAAGGGCCAGCCGGCGCTGGTCAATCGACAGAGCAGTATTGGAGTGAGTTTCTACAGTATAGTTGTGTTGCACCACCTTTACTATATCTCTGCCATCGAATCTCGTTCACAGCACAGCGTAT

 

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