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CRIMIC CR00327

Gene BubR1

CG7838

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:5973328..5973335	42A6		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	no band	GGTCAGTGATGACGTTGTGTTGCTAAGC	GGTAATAGTGGATTTAGTGGTGGTACCGC

Images

Construct Design Sequences

sgRNA sequence	TAAGGTGCACCTGATAGTCAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCTCCTGCACAGACGAAACT	TCAGGTGCACCTTACCTTAATT	AGGAAACCGACTCGTTCTCT	GACTGTCCTACACCAGTGCC

 

Amplicon 5'

Amplicon 3'

CCTCCTGCACAGACGAAACTCTGCGTGGCATGATGGATTTTCTGAAGGAAGAGGGAAAGAAGCTCTTGCGGCGTGGCATCTTGTTTCCGGGCAAGCAAACGGTTTCTTCCATGTCAAAGTCACCATGAACTGTCACATTTCGTTTTTCCATCCATTTAGCTGCCAACAACTCCTCTGGCTGGTACTCCTTTTGCTCAACTTCGTTGTAAATTTTGTCAAAAACTACCTGAAGGCCCGGTACCAGTGGGGGTTTCCAAAACTTGTCTAGCTCATCGAGATCTTGTGGCTTAGAATATCTGGCGAAATTCGGATAGCAGCGAATTCCTCTGATCACCCGTCCAGTGCCCCACCAATCATCATTGCGATGGATAAATGGATGCTGTGGGTTGCGATGCTTCAAAAAGGAATAGGCTCGATACTCTTCGGGCGAGAACTCGATGTTGGGGCGGGGGTAGAGGAGACACTTGTTATAGCAAGGCAGTCCACTTGCGTTTGGCTCGTCCTCAATAGTAACTGGCGTCACCCACGGTTCCTGAGGCCTGTTCTTGGCCACAAAGTTTGTCGGAAATCTAAATGGTTGTTCTAGACGACGCTCGTAGTTTGTAATAGGTGGCATTGCTTGTTCGTCAACGTGTATATCAAAGCCCAGATCCGGCGAGGCGTTACTGCCGAAAATTTTTCCATGTTTGTGGCGTTTAGCATTTGCCTTATTCCAGGCCACCGGTTCTTTTATATTCTCCTGACTGCAAGCCGAGTCGATAATGGATCGCAGGCTTGACTTCACCTCCGTCTCAGAATTAGAGATCGGAATATTTCCCTCAGAATTTTCGTCATTAAACACCTCAACGTTCCGACCTACATTGCGCCTACTGGTGGTGGTTGAAGGCTCGACCTGCAGAATGACAATGTATATTTTTAAAGAAATTTTATTCGATTTAAACCTTTTTTATTACTACTGTATATTGATATAAAAGCTTAGGTGGATTTAAAATCCCTTAGAAAAAATCGAGCTTAACGGCGGGATCTCCAAAAAGTTGGACAGGTTCACCCTAAGCCGTTTAATAAAATACAAATACAAATTAAGGTAAGGTGCACCTGA

AGGAAACCGACTCGTTCTCTATCGTTCTCTGTTGTTTCTGAATTCTAGAGAAGATAGTCATTAAACATGCATCAAACATAAGTTATAAAAGCAAAATTATACTTGAAACCGATTTCTCTAGCCAACCAAGTTCTTTGTAAACATGAAATAAAAAATCACCCACCTGAACCACGCCTGGGAGGCCGCTTTTAACTGCTGCTCCGGTACGGATGCTGCCCACGGTGATGCTGTTAGAGATCTGCTGCCGGCGACCATGAAGTGATTGCAGTGCTAGCCGTCGCTCGTTAAGTTCCTGGTTTACTGCGTTGGCCTCTTCACCAGTGCTGTAGAGCATGCGCTGGCCCACCGTATAGCCAAAGTGGTGGTGGGCCTCCTTCAGATCCTCTAAGGGCTCTGCGCGGCACTCTAGACCTAAGCGAAAGATCTCGTCGGCCTTGCGCATGTTACCGCTCAGGTCGTAGCTGTAGGCCCAAGCAATGTAGAAATCTGCCAGCATTGTTCCGATCCCGTTGTTGTACATCTGCTGATAAAACTCGATCTTGTCCTTCTGTTTCTCCATCTAAAAAGTGACGAAGTAAAATCGGTGGTTGAGTGATCGTTTAAAATATCATCGGCTACTCACGAATTTAATGAAAAGCTTTATAAGCCGCTTGTCCTGACGGTAGCGCTCGTCGTCTTCAAATTTGGTGAGGCACTGGTGTAGGACAGTC

 

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