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CRIMIC CR00329

Gene gsb-n

CG2692

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:25050429..25050443	60F2	FBti0199351	78890

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACAAGGAACGACAGCCTACTGATCA	TCCCAATCCCAATCCCAATCCCGAT

Images

Construct Design Sequences

sgRNA sequence	GTGAATCGGTGGAGCTGGTCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCATAGGGAGGGGTAATCGC	GATTCACGCCTGCATGGATG	CGGCTAGTGGGCAGTATGC	CAGGACAAGGCCGGGTAAAT

 

Amplicon 5'

Amplicon 3'

GCATAGGGAGGGGTAATCGCGGTCAGGTATGAAATCAAGACACAAGAGATGCCTAAAGCATATTCTAATTAAGCGTGAAGCTCACCTGGAGGCAGTTTTTACGCCTACTGCCACGCAAAAGCGAGCGCACACAATATAAGGCCTATAACACCGAAAACAATCAAAATCCCGATCTAACGGTTTTTGTTATTGTGAGGTCATACAAAAACAGCAATGTGATTGCATATTTTTGTCATTCATAAAATTTTAAATGCAGTTTTTAATTTTTTATATTAATAAAGCACTAGTCCAGCACTCTTCCCATAAAAAAATAAGAAGATAGTAAGCTAAAAACTTAGTTTATTAAAAAACTACTTTTGCGAATTAAACCATCCTAAATTTCTTTCTGTGCATATTACACAGTGCGCATCTTTTGGATTTGGAATGAAGTGATGCCGACTCTGATCTATAGTCCGATCGTATCGATCGCCCGAATCCTAACGCCCCATAAAGTGTGGCTAATTGCGGTTTCAAATAAAGAGCGGGACGATGCCGCCAAAAGAGGCTGGCGCATGAATACACTAGTGCTTGTATTAATCCTAAACCCTACACTCCCTCCACCGTCGAGAAGTTTTGCCGAAAGGGAAAATCAACTTCAAGACGGCCGTCATCCATCCATGCAGGCGTGAATC

CGGCTAGTGGGCAGTATGCCGAAATCTTTTGCCAGACTTTTGGTCTCTGTGAATGAGTCATATTGGCGTAGGTTCAGTAATGAGCTGAAACACAATTTCCCCGTTGGACTTCGCTTTTGCCACATAAGGTGTGGGGAAGTGTGCTCATCTTTTAAGCTTAACGCAAATCTGAAGCAAAATAATATCGCAGTATTGGCAAAGGTGGCTAGGTTTGTAAGACGGCACATTAAAACAGAGAATAGATGACAAGGAATATGTCGTTAATTATAAATTTTGTTAATCTAATAAACGAAACGAACTTATATGCGAGCCATTTTAATGTTTTGATGTAGGACCGATTCTTTCACGCCTCAAAACCGGAAGCGAAGTCCCGACGGCGAAGAATCATATAAATATATTCTCGTATAGCACCTTTTCGCCTCAAGGACAAAACACAAACGATGCCGACAAGGAACACGACAGAAGCGGTGTCTTTATGGTTTAATTGATTTCTTAACGGTTTGGGTGGCAAGCGAAAATTTGTCGTCGCTGTTGTTCAACAATATTGTCAATGAGCGGGCAAGTTCAAACTGGGTCCTTGGTCAGTTTGGGCCATCTGTGCAGCTAGTCAGCCGACATATGCAAAAGGCCAGTAGGTGTCATAGGCAACGTGCCATAACAAAGCCAGCGGGCGGTACGAAACTGCCACGTGACTTTTCGGAAAACGGAAGTAAACTGATTCGCATTCGGTGCGTATTTTGACCCTCTTCTATCTGGACATATTGGCATGCGGTTAAGGCTACCGGCAGGTCCTGCAAATCCTCGCATCGTTTGTTTTGGTACATTTTATTGCTTCCTGCAACCTTGCAGGTGCATCTCGGTACTCACCTTTATCAGCTTTTCGCGTATTTCCCAGCTGAATATGCTGGGGTTTTCCTTTCGCAGCTCATCGATCCGCGTTTCAATTTCGGGAGAGGTCACCTTGGGCTTAGATCCACCTATTACGCCCGGTCTAATAGAACCCGTCTCCTGGTATCGGTTCAGAATCTTCGATACGCAGCCGTGAGACACGCGGAGCTGGCGCGATATTACACAAGGCCGCACTCCACTGGCCGCCATTTCCACGATCTTCAGTCGAATGTGATTGGGCAACGGACGTCCATTGATAAAGACGCCCCCAAGCTGATTTACCCGGCCTTGTCCTG

 

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