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CRIMIC CR00332

Gene Gk1

CG18374

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:204684..204688	61B2	FBti0199566	79240

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	AGATCAGCTGATACTCTTTAGCCTGCG	CCCGCCCGTAAGATTATACATTAGCC

Images

Construct Design Sequences

sgRNA sequence	TGATATAGGCACGGCCGAGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGGGAAACGCCGTAAGCTA	GGCCGTGCCTATATCAAGC	GTGGACCTTTCCGTGCTTCA	TCGTTGGGGCTATTGACGAG

 

Amplicon 5'

Amplicon 3'

CTGGGAAACGCCGTAAGCTAGGGGAATGGTAAAGAGAGCAGCGCAGTAAACAACTAGGTAAAAATAATTGATAACATCTTACGACGTAACGATTCGAAAATTTTCCACAGCCGGCGACCCGTTTCAATGTCAACTCTAGTTAATACCACGAGACGCAGCATTGTGATCGCCATTCATCAGCAGCTACGCAGGATGTCCGTCCAAAAGAGGAAGGATCAAAGTGCGACCATTGCTGTGGGTCAGATGCGCTCTACCAGCGACAAGGCGGCTAATCTTAGCCAAGTGATAGAGCTAGTGGATAGGGCCAAGTCACAGAACGCCTGCATGCTCTTTCTGCCTGAGTGCTGTGACTTTGTGGGCGAGAGCCGGACCCAAACTATTGAGCTCTCCGAGGGCTTGGACGGCGAGTTAATGGCGCAGTACCGGGAATTGGCGAAGTGCAACAAAATTTGGATTTCCCTGGGTGGCGTGCACGAGCGGAACGATCAAAAAATCTTCAACGCTCATGTTTTGCTCAACGAGAAAGGGGAACTAGCAGCAGTATACAGAAAGCTGCACATGTTTGATGTTACGACTAAAGAGGTTCGCCTACGCGAATCAGATACAGTTACGCCGGGATACTGCCTTGAGCGCCCAGTGAGCACTCCAGTTGGCCAGATAGGGCTTCAGATTTGCTACGACCTGCGTTTTGCTGAGCCGGCGGTGCTGCTCAGGAAGCTGGGTGCCAATTTGTTAACATACCCATCCGCATTCACATACGCAACCGGTAAGGCGCACTGGGAAATCCTCCTGCGGGCCAGAGCCATAGAGACTCAATGCTTTGTGGTCGCTGCGGCTCAGATAGGTTGGCACAACCAGAAGCGACAGAGTTGGGGCCACAGCATGATCGTTAGCCCCTGGGGAAACGTACTGGCTGACTGCAGCGAGCAGGAGCTTGATATAGGCACGGCC

GTGGACCTTTCCGTGCTTCAATCTCTGTATCAGACCATGCCCTGCTTCGAACATCGTCGAAACGACATCTACGCCTTAACAGCCTACAACCTTCGTAGCAAAGAACCAACCCAGGATCGACCATTTGCCACTAATATCGTGGACAAGCGCACAATTTTTTATGAGTCTGAGCACTGCTTTGCATTCACCAACCTGCGCTGTGTTGTAAAGGGTCACGTCCTTGTGTCCACTAAGCGAGTGACTCCGCGCCTTTGTGGCCTCGACTGTGCCGAAATGGCGGATATGTTCACCACTGTGTGTCTGGTGCAAAGATTGCTTGAGAAGATTTACCAGACAACTTCCGCCACCGTCACTGTGCAAGATGGTGCACAGGCGGGACAAACTGTTCCCCATGTACACTTTCATATAATGCCCCGCCGTCTGGGAGACTTCGGGCACAATGACCAGATCTACGTGAAACTAGATGAGCGCGCCGAGGAGAAGCCTCCGCGTACCATCGAGGAAAGGATCGAGGAGGCGCAAATTTATCGCAAATTTCTGACGGACATAAGCTAGCTTTTTACCCCACCCCTTTTCCTGCCATGTGATTTATGAAGAACATATAATAAAGAGATAGATTTTCAATCGGCCATTTCGGAATAGATCAGATGAGGGAATCGATTCATCTGAAATTGACGTACCTCAACCTTCCCGCCCACGGCCACAAGTTTCTTGCAGGCACCGGTGATGCACTCGTTGACCGTATTCACTATGGCCATGGGATCCTGCTCGCACCATCCCTCCTTCTGGAAAATAGACTCCACTTCAATCTGATGAAAGCACACAATTTCGTCAGTGCCCGCACGAAATATAATAAATCGCGCGGAAGTGGTTCCCTCGTCAATAGCCCCAACGA

 

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