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CRIMIC CR00333

Gene Actn

CG4376

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:2033891..2033907	2C5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	short band	CGATCTAAGCTGAGAACGAGAAATGCAAC	ATCAGTCTGCAACACACACACACGCA

Images

Construct Design Sequences

sgRNA sequence	AGTAGGTGGCAATTGAGGTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAAGCTCTAGCAGGTCTGGC	GGCAATTGAGGTGGGGACA	AGATCGGTGTTTCCAGCTTT	TAGCCGCGGTTCACATTCAT

 

Amplicon 5'

Amplicon 3'

AAAGCTCTAGCAGGTCTGGCAGTCTGGCAGCGGCGGCAGCAACGCACTCGCTCTTGCACACCCATTTTTAGCCGAGTGTAATTTGAGCGGCCCCATAGCATGGACTCATTCGCACTTGGCCAGGCCGGGCCAGGCCAGCGGATGGAGGAGTGTGGCAACCTGGCAACCCTGGCCGCTGATCCTCCTCATTCATTCACACCTTTCGCATACATGAGTCACGCGGCGGAGCCTCACCACCCACTTCCACACCCACACCAACGCCCATTCCCTCTTTACACCTTTTGTTGCGTTGTTGCGATCAATGTAAACACAAAACAGAAACAATTTCGGTGGCTGTTTTCTTTCTTTTTTCGGCTCTCATTATTCAGCAGCTGACGAATTCCGTTCGGTAATTTACGGTTACGAAATACGTACATATATCGGACGAGAGGAAGAGAGACAGTCGGAGAGATGAAGAGATAGATGCCACGCATACATGTCCGATCATGTCAAGCTCAAGTCATTCGGCGACCGCCAAAAATCTTCATATTTTCGCTTTATTTTGCATTTTTGTTCGACTTTTTTCGATCTTTGTTAATTATTTTCAGTTTCGTTAGTGCGCCAGCGATTTGTCATTGCATTTTCGCCATGCTTTATGAGTGTGGGTGGCTGATGAATTTCGGATACAGATACAGAAACAGATACAGATACATGGGTATATTCTGAATCCGAATCGCGTGGAAATGTGGTGCATAGGAATGCTGTCTATTTATAATTGTCAATTTCATTTCACCACCTAGGCGTCGTGTCAAAAACACAGCCTTGCACTACATTTTGGCTGGCAATTTATAGAATTATGTATATAAATACTATATCTCTAGGTTAGAGCTCTTGGGTATGGAATGATATGGGCAAGGGTGCGGGCTCGACACCATCCTCGATACACTTGCCTTTAGGCTATCTAACGTTCCTTTCGGCCTGTTGGGCTCTTATCGCCTACGACCTTCAGGTTTGCTAGATGTCCCCACCTCAATTGCC

AGATCGGTGTTTCCAGCTTTATCTAAACAGTGGCAATTAGTTGCTGTTCTTCTTCTGACGCCATTTGTATAACGGTATGCTTCCGCTGGCACACAACGACAAACAACAAGCAAACAAACAAACATGCAAACAATCTGAACGACGAAAAAAGAAGATGAAAGGGATGGCAGGGGTAATTGATTGGAAACTGTTTGGAAAATAGATTGATTGAGGGTTTCATTGGGTGCTCTTAGGTGATGTTCAGATCGCTCGCCCCCAGACAACAGTGGTTGGTAGAATCGAATGAGATTTTCGATTTTCATAATCATCAGAGATCGAAGGCTTTTGGATATAGGTTCTATATGTACTACATATATATGTATGTATAGTTCCTGTACAGCAACAAACTATTTTCGTGCTATCTTCAAACATTGCTAGAACAACAAGAGCCATGAAAATCAAGAAAGAAATATTCTATTTTTAAATCGGGCCGCTGAGCTTTTTTGTAGTTGTTGTTCTAGCTTTTTTGTGTTAGTCCCATATTGTTGTTGTGCTGCTTGTGCTGTTGGCTCGGTAAAATATTGAAACATAATCGAAAAATATAGAACCCAGCTAGACCAAAAAGCGTATATCCAACACATATGAATGTGAACCGCGGCTA

 

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