CR00334

CRIMIC CR00334

Gene csw

CG3954

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:2108920..2108942	2D2	FBti0199352	78891

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAACTAAAAGACGTGATTTCTTCCAGCGG	TTGCTATACGGCAGAATGGCGCTCT

Images

Construct Design Sequences

sgRNA sequence	GCGGGCAAACTGCTTGCGCCAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAGTCGAGCACTCAGTCAG	CGCCAAGGCAAAGCAGGC	GAGCTTCAACCAGGTCAAGC	GTGGGCAGCCGTATGTAGTT

Amplicon 5'	Amplicon 3'
CCAGTCGAGCACTCAGTCAGTCAGTCAGTCAATCGCTCAGTTCGTTCAGTGGATGCGCCATCGATCGGTACATATGAATACATATCTACGAATAACAGTCGAACTAATTTAGTCATATATCCTAATAACCATCCACCTCTTTCTCTCCGCAGATGGTTTCATGGCAATCTTTCCGGAAAGGAAGCGGAAAAATTGATCCTGGAGCGGGGCAAGAATGGTTCGTTTCTCGTCCGTGAATCTCAGAGCAAGCCTGGCGACTTCGTCCTTTCCGTGCGCACGGACGACAAAGTAACGCATGTCATGATTCGATGGCAGGTATGTTGAATACGTAACCCCAACTAACCAGATTTTGTAGCCGCCCCTTGCCCCGCGCCCCTCGCAGGGTGCAAAATGCAAATGCAAATCGATGGCAGTGCAGCAACAAAGTCGCACTGGCCATAAAGTTCCTCATAGCGCGACCTTGGCTCTACAGTCGAGTCGTATGTACATATGTATGTGCACATAAACTCTACAGTTGAGCCTAACTAACAACGATGCTTGCTCGAGCGGAGTGGCAAGAATATTATAAAATCAATTGACAATAATATATTTAAACTCTTTCATCTTGAACGCATTTGGCTATCCGTAATTATCCGTTAACATTATCGAAGGCCTACTGTATAAACTCCGAAACATGCCTGCTTTGCCTTGGCG	GAGCTTCAACCAGGTCAAGCAGTGCAGCAGTTCCAGTTTCAGCGTAATTCCCCTAAAACAATTTTTCCACTATTACTATAACTTTAGCCGTGAGCGCGGTTTCTGCGGTTTGCGGCTGCAATTATGCCATTTACATGTCTAGCTGGCCTTTTTCCCATTTGACATTGAAGTCACGTTTTCGATTTTATTAGCTAATCTCTCGACGCTCCATCGAACTCGGAGATAGAGACTCTCCACCACGTGTCCCCCCATTAGAATGCCACGATAACAATAGAGTGATTTCCTGATTGCAGGACAAGAAGTACGACGTCGGCGGCGGGGAATCCTTTGGCACCTTGTCGGAACTGATCGATCACTACAAGCGTAATCCCATGGTGGAGACGTGCGGAACCGTGGTGCATCTGCGACAGCCATTCAACGCCACACGAATCACGGCGGCCGGCATCAATGCCCGGGTGGAACAGCTGGTCAAGGTGAGTTCCCTCTCTCTGATAGCAGTGATTCTGGAGCGCACTAATCCCGAGGTGGCTTTCGATTCCAGGGAGGTTTCTGGGAGGAATTCGAATCGCTGCAACAGGACAGTCGGGACACATTCTCGCGCAACGAGGGCTACAAACAGGAGAACCGCCTCAAGAATCGCTACCGCAACATATTGCCATGTGAGTATAGAAATCCACTAAGATCCGTTAGGACTCCCAAAATCCTGACGGTTTAAAACAGTTATTTCTAACTAACTAACTAACATTAACTAACAAATTTCTAACCTATTTCCCTGTCCGCTTTCCAGACGACCACACGCGCGTCAAGCTGCTGGACGTGGAGCATAGCGTGGCCGGAGCCGAGTACATCAATGCCAACTACATACGGCTGCCCAC

Amplicon 5'

Amplicon 3'

CCAGTCGAGCACTCAGTCAGTCAGTCAGTCAATCGCTCAGTTCGTTCAGTGGATGCGCCATCGATCGGTACATATGAATACATATCTACGAATAACAGTCGAACTAATTTAGTCATATATCCTAATAACCATCCACCTCTTTCTCTCCGCAGATGGTTTCATGGCAATCTTTCCGGAAAGGAAGCGGAAAAATTGATCCTGGAGCGGGGCAAGAATGGTTCGTTTCTCGTCCGTGAATCTCAGAGCAAGCCTGGCGACTTCGTCCTTTCCGTGCGCACGGACGACAAAGTAACGCATGTCATGATTCGATGGCAGGTATGTTGAATACGTAACCCCAACTAACCAGATTTTGTAGCCGCCCCTTGCCCCGCGCCCCTCGCAGGGTGCAAAATGCAAATGCAAATCGATGGCAGTGCAGCAACAAAGTCGCACTGGCCATAAAGTTCCTCATAGCGCGACCTTGGCTCTACAGTCGAGTCGTATGTACATATGTATGTGCACATAAACTCTACAGTTGAGCCTAACTAACAACGATGCTTGCTCGAGCGGAGTGGCAAGAATATTATAAAATCAATTGACAATAATATATTTAAACTCTTTCATCTTGAACGCATTTGGCTATCCGTAATTATCCGTTAACATTATCGAAGGCCTACTGTATAAACTCCGAAACATGCCTGCTTTGCCTTGGCG

GAGCTTCAACCAGGTCAAGCAGTGCAGCAGTTCCAGTTTCAGCGTAATTCCCCTAAAACAATTTTTCCACTATTACTATAACTTTAGCCGTGAGCGCGGTTTCTGCGGTTTGCGGCTGCAATTATGCCATTTACATGTCTAGCTGGCCTTTTTCCCATTTGACATTGAAGTCACGTTTTCGATTTTATTAGCTAATCTCTCGACGCTCCATCGAACTCGGAGATAGAGACTCTCCACCACGTGTCCCCCCATTAGAATGCCACGATAACAATAGAGTGATTTCCTGATTGCAGGACAAGAAGTACGACGTCGGCGGCGGGGAATCCTTTGGCACCTTGTCGGAACTGATCGATCACTACAAGCGTAATCCCATGGTGGAGACGTGCGGAACCGTGGTGCATCTGCGACAGCCATTCAACGCCACACGAATCACGGCGGCCGGCATCAATGCCCGGGTGGAACAGCTGGTCAAGGTGAGTTCCCTCTCTCTGATAGCAGTGATTCTGGAGCGCACTAATCCCGAGGTGGCTTTCGATTCCAGGGAGGTTTCTGGGAGGAATTCGAATCGCTGCAACAGGACAGTCGGGACACATTCTCGCGCAACGAGGGCTACAAACAGGAGAACCGCCTCAAGAATCGCTACCGCAACATATTGCCATGTGAGTATAGAAATCCACTAAGATCCGTTAGGACTCCCAAAATCCTGACGGTTTAAAACAGTTATTTCTAACTAACTAACTAACATTAACTAACAAATTTCTAACCTATTTCCCTGTCCGCTTTCCAGACGACCACACGCGCGTCAAGCTGCTGGACGTGGAGCATAGCGTGGCCGGAGCCGAGTACATCAATGCCAACTACATACGGCTGCCCAC

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