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CRIMIC CR00338

Gene ktub

CG9398

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:21119359..21119382	57C1	FBti0199353	78892

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCATTCTGTTTAGTACGTGCTCACGGCT	ATGCTTTCGATGGTGGAGTTCACCTC

Images

Construct Design Sequences

sgRNA sequence	CCATGTGAACCTGGAACAGCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACGTGCATGAACAGAACGG	ACATGAGGATGTCACCGGTG	GGATCGAGTGGCCTATCTGG	ATCCGCGAACAGGTTCTTGT

 

Amplicon 5'

Amplicon 3'

CACGTGCATGAACAGAACGGGTTTGACCGCCGGCCCGACGAGACGGATTTCCCTATCGCGCCGTGACTTCGATATCACGGAACCTTATCAACTCGGGTACTCGAAGATGTGGCCACTGCCATAAATCTCGTCACAGTGATTCCAGTGCCAGGAGAGGTGGTGGTCAGACGCCTTAGCACCAAAAAAACCAGAACACAGTTCCAATTCGTGCGCCGTCGAGGGCAGACATGTCGGTGTCCTGGTCGAAGACTTTCGCGGCATTCACCGTCGCCTGGCTCAGTGCCCTGTGCCTGCTGCCCGTGGAGACATTCTGTGACACCTCCTACTACGAGAAGTACTACGCGGCGCGGCGCTATGTGCCCCTCAAGCGAAATGCTCCGCTGGTCTTCAACTTTACCCTAACGCTGCCGGATGCGGATCAACTGGCGTCCTTCGAGCGGCCCGCCCTGGTGGGCAATCTGCCAGTGCTGGGCGCCTGGCAAGCGGGACGCGCTGTGCTCCTCAACCGCACTGCAATCCTGAATGTTTGGAGCGCCTCCGTGGAGATACCGCAAAACAGCAGCGTTGAGTATCGCTACTTTGCCGCCGCCGTTGGCCAGAGTGGAGCCGTGCAGATTCGCCGCTGGGAGTCGCATGTCCAGGCAAGGACCTTTAATACCACCAAGTTCAGTGGCAATCGCAGCGATGAGTTCGGACTCATTGGCAATGAGCGCCAGTTGTCGCGTGGCTGGCTTCAGGACTCGGGCAACATTCTGCAGTTGAAGCTCTTCCGCGAGGCTCTGAGCTTGGAGGAGCAACCGGAGGTGGGGCAGGAGAAGCTGCGTCTGCGTTTGCAACCAGTAGATCCAAAGACTCTAGCGCCCATCCTAAGTTCGGCACGTGCCAATGTGGAGTACGTGCGAATGGCGTACGGTGACAGTAAGCTGCGGGTGCAGCCGGAGTACGGAGTGCCCTACGCCACCGGTGACATCCTCATGT

GGATCGAGTGGCCTATCTGGTGGAAGTTTATGCCGAACAACCGGATGAGGACCTGGTCCGTCTCCTAGGATACTCGCACCTGCTGCACACCTCGTTCTCCGGCACTGAAGGCGATGTGTCCATCGACCTTATTTCGCCGCTCTGGCAGCGCGTGGTGGGTCAACTGCGTATTGAGTACCTGCACATTCGGCCCATGGAGAAGGATGGCTTCGATCTGCGCACCAGCTTCGCCAACTACTGGCGCTCCAATTGGACAGCCCTGGAGATTGGCCATCGGGGATTGGGAAAATCTCTTACGGTCACCACGAATGCGGCTCCGCTTATCGAGAACACGGTGGCCACCATGCTGGCCTCCTCTGAATTGGGCGCTGATCTCGTGGAGTTTGATGTGCAGCTGACCAGCGACCTGGTGCCCATCATCCACCATGATTTTTCCATTCGCGTCTGCATTGACTCGAAGACCCCAACGAGCAAAGATGATTTGACCGAGGTGCTGCTAAAGGACATATCCTATGAGCAGCTCAAGGATCTGAAAACCTACCAGATCGTGGGCAACAAGATTGTCGAGTATCCGGCGCACAACAACGTGGAACCGCCGGAGCAGCGACTTTTCCCCACACTGCAGGACTTCTTTGAGCGGGTGAACCTGTCCACCGGATTCGACATCGAGATCAAGTGGCCACAGCAGAGGGCCGATGGACTGTTTGAGAGCGAACAGACGCTGGACAAGAACCTGTTCGCGGAT

 

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