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CRIMIC CR00341

Gene Surf4

CG6202

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:15345439..15345450	88F1	FBti0199355	78894

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCTATGGGCCCACTAATTGTTCAG	GCGCGAATGGATTTGGAGTTGGGTT

Images

Construct Design Sequences

sgRNA sequence	AGGTCGAGTTCCGTAATAGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTTGCCATACGCTCAGCT	AGAAAGCCAGGTGAACTTGCT	TTACGGAACTCGACCTGGCC	TAGGCCCCATCACGGCTATA

 

Amplicon 5'

Amplicon 3'

AACTTGCCATACGCTCAGCTCAAAGCGGATCAAAGTGATGAACATGAAGGCCAGCAGGATGCGGCCCGCCAGCTGCATGAAGTTCTTGGGCTTGTTTTCGCCCATCGATGGCACGCCGGCAAACAGGCTGCGCCCCTCTATCCTGGCTTCCGCCAGGACGAGCAGTAGGGCTCCGATGAGTGCAAAGTTTCGCAGTAAGAACTGGAAGTCCCACAGAATGGAGTAGGCAACGGTCTGCAAACAGAGGATCGGGTTAACGTCAAGGCTTTTGCTTCTGGCTGATATAAATGCAGACTCACCTGCAACACCACAATGAAGAAAAGCAGTCCCACCGCTATGTCGACCTTAAATCTGGCCATCACCATGCCGCAGCCGCCCAGTTGGCCCAACAGGTTGACGAGCACAAAGACGGTGGCCAGGAACTTGCCGCAGCCCCAGCTCATGTCCATGTACTCCCGCTGCTCGTTCCATTGGATGTACATGCGCAGGCCGTCCTCGAAGAACGTGGCGATGAGGCACAAACGGGCCACTGTGGGCAGCACATTTTTGCCGCGCTTGATGACCTGCAAAAATGAGTGTTCGATTGGTTCTGGTTGAGTTGAGAGCAATTGGGATGGGTGCTGATATGGTGATAAGGGGACTACTGACAAGGTGATAAGGGATTTAAATATACAATGCCGTTGAGCGTGGCTTGCGCGTAGTCGCTAGAAATCGCCCGCTGGCTGTGACGTGTCTCTAAACGCTAGTATCTCTCGATTGCTATCTGCCAGCTAACGGGTTTTGGCCCGGCTGTCAATGTAACTTCATACATGTATGTATGTAAATCGCGAGCAAGTTCACCTGGCTTTCT

TTACGGAACTCGACCTGGCCGCTGGCGTCCAGCTGCCCGCTATTTCTTTTTCAACACTTTCACTTGCGGAGACAGCTCTCTCGCACACACACACAGACACATACGCATCAGCACACAGGTAGAAAATAAGCAGCGAGAGAGAGAGTTTTCCCTGCGGCAGACGCAACAACTGTCAATTGCGGTACTGGAGTACAAGGCAAACCCATCATCTGGGTTGCACCCAGATTCTTATTGGAAGCAAATGCCCAGCACGTTGCCCCCATGTCAGCGGATCGTGATTATATGCAGCCAAGACCCTCGAACTTTGCCGCGTTTGGGTGACGTTGGCAGTTTTCCCAGTTTGCAGCTGCCAGGGCTTCCCCGCAAACGCCATTTGGGGCGCATCGGGAAGACGCTTCCTGCGGTGGATATGGAGTCCTGGTGGACTTCTTTTTGGCAGTGGAACCTAATATATCTTACCTGTTCCGCCACATCCTCCGTTTTCGCTATGTACTCGTTGGGTATGCTCATGTTGGCTGCTTTGGGCTGATCCGATCTCTCACTTTGGGGCAATCGTCTGTGGAGACACTTTGGCAATTCGTTTCTGACTTCTTGGGCGTCGCGTCGCTCTCCAAATTGTTGTTAAGTTACCAAAAAATCATGAATTAAAAGGCAGGAACTTAAAAACACACACGCACGACAAAGTATAGCCGTGATGGGGCCTA

 

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