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CRIMIC CR00344

Gene RpS5a

CG8922

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:17144499..17144513	15E5	FBti0200542	80581

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	AGCGTTGATCCTTACAGGGTTGTGGT	CCAGATCGATGGAGTATCGCAACTATC

Images

Construct Design Sequences

sgRNA sequence	GTTAATCCGAGTGCCGAACGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTAACGGTTGGACTTGGCGA	CGGCACTCGGATTAACCCA	ATCTGGTCTACAAGCGAGGC	CATCATCAACTCGGGACCCC

 

Amplicon 5'

Amplicon 3'

TTAACGGTTGGACTTGGCGACACGCTCCAACTCATCCTTCTTCTTGATGGCGTACGAGTTGGAAGATCCCTGGAAATACATTTAAACACAAGTTATTAGTATTGGCTATCAATGTTTTTCTAGATCTTTTATATGTTAATTGCGCTGTTATGTTACTGTTACTGCATTGTATGGATTCATCGCTTCTAAATAAATAAATATAAAAAAACAAGTCCCTAACTTATTTAAAGCTAGTGTTCCTTTACGTTTCTCATGTGCATTGAACTAAGCAATATTACAAGCACAAGGATAATAAAGGATGGAAATTAATCTGGTCCAAAAGCGAGACTACATAATTAAACCACCATGCAGAATTAGATTGGACCTATTATTAAGCTCTATATACCAACTCTTAAATAGTTTGCTAATGTTCTTTTACGTTTCTCGTGTGCATTAGACTGATATTGTCACAAGCACAAGGATAATAAAGGATGGTGATTGTTCTGGTCCAAAAGCGAGACTACATAATTAAACCACTATGCAGAATTAGATTGGACCCCCGCTATCCTAATCGAATCAGTTCCTAAATGCTAATTCATAAAAGTGTTCTTTTACGTTTCTCGTGTGCATAGGACAGTTAAGTGGTCACTAGCACAAGGATAATAAGAGATTTGGAGTTTGATCTGGTCCAAAAGCGAGACTACATAAATAAACCATGATGCAGAATTAGATTGGACTAGTTGAAATGACTAGCTGTGTCACTAGAAGGAAGAAAAGAGCTATTTCGAAGCTCAATTCTTGGGTTAATCCGAGTGCCG

ATCTGGTCTACAAGCGAGGCCAGGCATTTTGGTAGTACAATGCCAAGCATTAGAGTAGACCACCCCATTCCCACTTTGATCTCGCCACAAGCCCAATTTACCCACCTTAGCAGCGTTGATCAGCTCATCAGCCAGGCACTCGGCGATGGTCTTGATGTTCCTGAAGGCAGCCTCACGAGCTCCAGTGCACAGCAGCCAGATAGCCTAAAGGAAATATACGGAAAAAAATTAATTAAGTAACTTTATGAACGCAATCACAGCAGCGTAAGAATTTGGGGTTCTTATCGTTAGTAAAATGAAAGTGTGTTATGCAATTGATAATGTCTCTTCGTTTCATACGCCAAATGTCGGCTATACTACACATTATGACGCTACGGATATTAGATAGATTTTCGTTCTGGCCAAGTGTTATAAGCAAGCAGTCTCTAAACGAACCACAAGCCATTGCTACCGGCCACCGTGAGCACATGCATCTGGAAGGCAAATCTTCAGAAGAAACCCACCTGGTTGACGCGACGCAGGGGCGACACATCGACGGCCTGGCGACGGACGGTACCGGCACGTCCAATACGGGTGGAGTCCTCACGGGGTCCCGAGTTGATGATG

 

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