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CRIMIC CR00347

Gene Lgr3

CG31096

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:25670605..25670619	96E8	FBti0199356	78895

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTGGTGAGTGAATGCGCCTCAATTG	CCACCCAGACGACCCATTGACTTATA

Images

Construct Design Sequences

sgRNA sequence	CAAGTGAGGAATGTGAGGCCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAACAACGTTAGCCACCTGC	AGTGAGGAATGTGAGGCCTG	ACACACAATGAGCTTAGGGC	GCCGGTAAGAAGGTGTCGAA

 

Amplicon 5'

Amplicon 3'

CAACAACGTTAGCCACCTGCCGCACGGTGTCTTCCTGGGGCTGCACAGCCTCCAGTGGCTGTTCTTGGTCAATAACCATCTGCACCACCTGCCCGTGGAGCAACTGCGATTCTTTAGACGATTGGAGTGGCTGGTGCTGAGCAGGAATCGTCTCACACTGCGAAATGTGCAGCTGCCGAAAATCCCGACGCTCTACGAAGTGTGAGTAAATCGAATTGGGCGCTCGCTCTTCTATTTGCTAGATTAAAAACTGAAAGGACTAGCAGCTACGCTGATGTATTCAAGCGTGAAATGCTGCTAAAAGCTGCTGGTGCAGTGGTGCTCATTGGGTTACTCGAGTATTGGGTGTCCTGGCGGATAGCTGGTTGGCCCTAAGCCGAGCCAACTGCTCGGTGATTCTGTTAAGGACTCACACAATGTTGTCAACGTGGTTGAACTCACTTGGCGACCAAAGTGTCCGTTTGATGTCCTTTAGTGGTTTTGAAGTTCACGATTTAAGGTTGCAACATTGAAGTTTTTAGCTCTCTTCAAAGGCTTACATCTACACATTACCAAGAAACATTTTTATATAAATTCTTAAAGAGAATCCTAGTTGCCCTGCATCAACTTTGATTATCTTCCGTCTGCCCAGGCCTCACATTCCTCACT

ACACACAATGAGCTTAGGGCTGTCTTTGAACACAGATCCGAAAATATACATATATAAACCAAACTAGGCCTACAGCTTAGCCAGATTGTTGGATGCCTGCATAAATATCTCTTTACTTTTACAGTTATTTGGATTTCAATCGAATCGAATACATTGGCGAGGAAACTTTTTCCCAATTGGATAATTTACATTTGCTGTAAGTAAAACAATCAACATTCACCCACTCGTCCCAAAACAAAAAAAAAAACAAAAAACATGGCAAAAGATGCCAGCAAGTGTCGAAAAGTCTATTGAAATATGTTGTACTTAGATTTTATGGGGCGTATGCGTAATTTTTACTCTTCGTTGCATTTCGCTTTCGTTCGTTTTCACTCGGATCTTTTTTTTACTTGTTTATTTCGAGAGATTTATGTGGGTTACATTGTTGCTGAGGCAGTAGAAAAGATGGATGGAGCATTGCGTATACGCACTGTTGACATGACACTGAGGTGGATATTCGATACTTCGACACCTTCTTACCGGC

 

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