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CRIMIC CR00348

Gene Plod

CG6199

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:11201107..11201122	68B1	FBti0199567	79241

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	short band	TGATCTCGATGTCCAGCGGATTTGCAA	ATTGATATCCTTGCAGGCAATTCGTGGC

Images

Construct Design Sequences

sgRNA sequence	CCAGCAAGACACATGGAAAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCTGGGACGCAAGATCACTT	TTGCTGGGATTCGGTCTAGT	GGTGCCATGGCCTACGATG	TCGAAGTGCGAGGCTTGTAG

 

Amplicon 5'

Amplicon 3'

CCTGGGACGCAAGATCACTTTCTTTTGCATGGCTCAAGGTGAGAGTTAGTTAGATGAAGTTACCAAGTCACCAAACATTGACATACTGACATTTTTGGATATTAGGCAATCCCCGACCGACCATTACCTGGTTTAAGGATGGCGCAGAGCTCTACCAACACCGATTCTTTCAGGTAACTTACGAAATTAATACTTTAAATATATATATAAAATTTTTACATTTACAGGTACACGAATCTCACATAGAAGCGAATATTGTTAAATCTAAAATGGAAATCGATCCAACCACTCAAATGGATGCTGGTAAGTTCGACAAATGATCATTAAATGTATACTTAGATTATATGTATATATTATTACCAATCTTTTCAGGTTTCTACGAGTGTCAAGCGGACAATATCTATGCCATCGATCGTCGAGGTTTTCGCACGGACTACGTTATGGTTAACTTTTGAGAAAAGCCCACTTTTCAGCCATTACAAAATCCTTTGACACAGTAAAGCTGAGATGTAACTATAAACTACAATGCTTTCGATACAATTAAATAAAAATATTTAAAAATACATTTTTGCGTTTCGGATTATGGAAATTTAAAGTCTATTTCAATCCGACCTTTCTGCTTCCTCGTGTGCATTTATGTGAGTATCTGTGAGTGGCAAAGTACATAGGTGTGTGTGCGCACAAAGCCGACAAATTGACTCAATTACCCAATTCAAGTGATTTTTTTGCATTTATTTTATTAAAATGTGTGAAAGCAAACCAAACAAACCGAGGCTTTTTGGTTCTAAAGTGTTGAGTGCTCTATAGCCTTCAAAAAGGCACAGTGGAACAAAGAAACACAAACATGTTTTTTAAAATAGAATAACAAGTTTACAAATCGATGTTCTTCTAGCACTAAGTAGTAATAAAATCTAAATCCATCTCATTGTATCTACAAATTGTTTTGACGAACCAAAATGTATAAACTATTATCTGCATATTCGGGAAAATATTTGAATACAACAATCCAGTTCCTTTAACTACTTTCCCTGAAAGCGTACTATCGTGCCATTGCCACTGTAAACTAGACCGAATCCCAGCAA

GGTGCCATGGCCTACGATGGCGAGCGATATCAGCAAGCTACCAAATCAAATCTAGCTAGGGAATTGAGTGAAATGTGGAAATGCGGCGATGAGAAATGTGAATCCAGAATAAAAAAGCCGCGAGCGGCGGCGAATTTCGAGAGCACACCAAACGGTTTAAATCCGCCGGACAATTGTGGCAAATTAGATGAGCCGAGCAGAGTGGTGCAGACTCTGGCCGAGTGGATCAGTCACCGATCAAACGAGATTCGCACAGCAACGCCAGCAGCAGCAGCAGCAGCAGGAAAATATAGCGACAGTGATCATCTCCGTCAGCAGCATTATCCATTATCCGGAGAGTCATGCAGGTTAAGTGCCTGATCGTCTGTCTCGCTTTGGGGCTATTAATGCTGGCCACGCCCGTTTGCGAAGCGCGCCGAGGGCGTGGACGTGGACGAACCAAGTCCAGGGTGCAGATCGGATTGCCAATTACCGGAAAATATCGCGATCCGGAGTCCGATCAGTACTACAATAATAATAATGTAAGTGAAGGTGAAATTTGCTGAAGTTCCCTATTAACTATTGCCTGTTTTAGGGCGCCAAAATCCTACAAGCCTCGCACTTCGA

 

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