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CRIMIC CR00351

Gene CG16758

CG16758

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:2501358..2501364	62E4	FBti0199357	78896

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTAAGACCTCCAACTAACCCGCAGAAT	TAGGTCCTTGTTGTACGAGTTCACCAGG

Images

Construct Design Sequences

sgRNA sequence	AGAACCAGGTGAATTCTTTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTCCTGGATCATATCGGCC	CCCAGAACCAGGTGAATTCT	TTGCTCGAACTTGAACTGCA	AGTCGACGCTGGCTACTCAT

 

Amplicon 5'

Amplicon 3'

GGTCCTGGATCATATCGGCCAATGAGCCTAGCCCAGAGCCGCAGATGATGCCGATCTTGGGGCGCATGCCGGACCCCTTGGTAATGAAGTCGGCGATCTCCTCGATGACCTCATAGGGATACCTGTCGAAAACCAAAATTAGTTTTACATCTTGTTTGGGTGAGTATTGCAGGGTGATCTTTGAACTAATAAAAGTGTAAACAATATACGAGTGAACTGCGATAAATTTGGGTGGGTACATGTTAACAGGCCAGAGATCCTTTTTATCTACTCGCCACGATGGTAAACAATGAAATGAAGAATGGAATTCCCAGAGGGAACAAAAAAGATACATTTACCAATTGATGAAGTTCCACACACAGGAATATAATTAGATTCAATTCACCAATCGAAGTTTTATCAAAACAAATAATTCATTTATGTACAATCAGTTCGATGATATGATAAGAAAACAACACAAATATTATACAGTGAGAACTCAGAAGACTAAAAACTTTAGAGATCATGGACACATGAATTCATTATCGAATTACATAGATATTCTTAAAAAGGACAAAAACAAAAATCGAATTTATGGATAAGAATATCTTTGAAGAACTGAAAGTGTCTTCTTGAAGATTCTTATCAGCCTTATGCCAATCACAGTACTGTGAATACATGTATTTTAATAATAAAGTAAATGACAATTGAACACAGAGAAACAGAACTCAGAAGAATTGGTCTACAAACGTGTCTTCGTTGAGAACCCGAAGGGCTCTTAGCTCTTCGTGCGTAAGCTCGCATTGGATTTTTCCATTTCCGCTGCAATTGTGGAAAAGATTTGTTGCTGAGTAGGTTGGCAAAGGTTTGTGATAAACGGATATGAAGGCATCCAGATAAGGACCCCCTATCATTTATGGTTTTTCTTGGCAATCAAAAGAATGTGGTAACATTGTCATGACTCGCTTGCTATTATCGAGTAACTTGAAAACAGCAATTAAGTGCTATTAGAGCCATAAAGAATTCACCTGGTTCTGGG

TTGCTCGAACTTGAACTGCAAGTCGAGTTATGTTAAGATGTTGTTACCAAAACTGAACTCACGACCTCCCACGTGTTTGTTTTCGGATCAGTTCAAATGATTTGATAAGCCGGGCATGAGACGATGCTAAGCATTATCAGTATTACATGGAAAAAACAAAAGCTGGCCTTCGAACAGTAAACATAAAGTGCGTCATTAAGACGGGAACATATAATCACGCATAACTTGCACTAATTGGGAACTTTATTCACAATTCCTTAATAAATTGGTCTTTCAGTAGCCAGACATTTATGGAAATTTGATCTAAACACGCGACGCGGCTTTTTCGCCGCTTCATCAATATTCAGCATTGTGATTCCATCGGAATGGAAACTAGTTTCGAAATTAATCAATGCGGCAACATGTTTTAGTTGCGTTCTTTATCGAACCATTGGCCAATTACCCGTATCTGAGGCAAAGATTACGAAGTGGAGGCCTATGTCAAGATCAGGGAACACACCCGTTTTGCGCAGCCAATCAGCGGGAGCCGATTTCCCAGTTAACTTGGCCATTAAACACTTACATAAGCAGTTGGCAATCTTGGCTTTTTTATCAGCAAAATGTTTGGTACACAGAGAAAATATAAATAATTAAAACAAGGCACTCTGAAGAGCGCCCAGCTGCTTAAAACGTGCTCTAAAAATATTAACTCTAAAATAATGAGGAAATTAGTTAATTAAAAAAATATTTCCTGAACACTTGACTCTTTCGAATACATATACGTTTTCTACTGTGTATTTCTACCTAGGCGAAACCACTCCATATAATCATATGCAGATTAAAGATTCTTCGAATGGGATTTTAATGAGTAGCCAGCGTCGACT

 

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