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CRIMIC CR00352

Gene LamC

CG10119

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:14575098..14575098	51B1	FBti0199568	79242

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	GTCAAGAAAATGTGGAATCAATAATGCACAGCAC	TTGGCGGCGGCATTCACCGAGT

Images

Construct Design Sequences

sgRNA sequence	CGAGACACAGGCAGAAAATGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACCCGCCTTTTAGCCAAAC	CGCGAGACACAGGCAGAAA	TGTCTCGCGCTCCAGTGG	CCGCCTAAAGGTACAGTGCA

 

Amplicon 5'

Amplicon 3'

CACCCGCCTTTTAGCCAAACTCTTAACTTCGTTAACGCATTGCAAAACGCACAAACAACAAATTGTGGCTATTATGAATTGAAAAAGCTTTGGGCCTCATCGCGCAGTAAATACCAAAAATAAATATGATAACGCAAACGACGCGAAACGGAAATCTCGCAAGTGGAAAAGCTAAGCAACCCAACTTTTGTTGACTTCCCACCAGAAACTGGTATTACAAATGGATCTGTAACTGAGTGCCATATGACATCATAGTCTTGTAACCTTCATTGAGATTTACGGCGGTATTTCGATTCTTCCTTTTTGATTTTCATTGGGTTTCTTTTGGTTTACTACTCACACATTAGCTGAGAACTAAACATTTGCATAAAAATGCCTAGAGAATTCTGGAAGTGTAAATGCAAAAAATAAACAACAGCGAAAGAGTCGAGAGAGTTTCCCATGTGCTTGGCTTTCAAAATGTGCTTTTGGCATGACATCATCGGCCTCGCTCGCTCTCAGTCTGCCCCGTTCTCTGTCTGTCTCTGTCACTCCCTACATATAAAAAAAAAACAACCATGGAAACTGAAGTTTGTATCTTACTAAAACTCTTTTCTTCTTACGAATAGCATGAAATATGAAAATTAAATATATTGCCTTTATAAATAAAACAATTTGTTATTGTTTTCGCTACATAACTACATAGCTGGAATTGAACTTCTAGGTCAACTGTACCTAAGAAATAAATATAAAATTCGCTTATTTCAATACAAAATACAACAAAAAAGTAAAAAAAAAAAAACAATTTGATAAAAAGTAGTGCATTTCTAGAAATCTCGACTGACTGTACTTTTACTTTACAAATCGAAGAAATTTCGCTGCTTTTCGTGCTGTGCATTATTGATTCCACATTTTCTTGACAAGGCTCCCAAAGTTGCCCCCATTTTCTGCCTGTGTCTCGCG

TGTCTCGCGCTCCAGTGGCCCGGCATGGGTACATATGTACATATGACATCACTGGCTGATAATACGCTGTTACCAATTCCGAGCCCAAACCTGAACCCGCCGTCTTGGAGCTTTACCTGGGCTTCAAGTCGTCGTTCTCCTCCCACAGACGCTTGATATCGATCTCAAGCTTGGCCTTCTCTTTGGCGGTCTCATCGAGCAGCTTGCGAGCGGCGGCCAGCTCCTTCTCATAGACCGCCTTCAGATTAGAGGTCTCCCTGTTGACGGTATCCTGGGCGAGATTCAGTTCCTGGGTGAGCCGGCTGTTCTCGTTCTCCAGGTTGCGCATGCGATCGATGTAGCAGGCCAGGCGATCGTTCAAATGCTGCAGTTCCTCCTTCTCCTGCTGCCGGCTGGTGCGCGTGGGCGAGGTGGGTGAGGTGGCGCCCACCCTTGACGAGGTGGATGCCCCGCCCACCGGCGTGGAGGTGGAGGCGCGCGAAACGCGTGTGTTCAATGTGACGCGGCGTGCTGACATTTTTGCAATGTGTTTTCTTTTCTTTTTGCGAGGTGCGAGTTGCTAAGTTAAGTACGTAATCCTCTCAAGTCACTGTCAATATTTTTGATTCTGAATTTTTTTTGCTTGGACGTCCGCCTGCTTTGACGACTAAAAATTGACTGAAACTTTGACTCGAAACGAACGGCTATCATCGACAAAATTAGTCGTCGTCGGCGGCGGCGGTCATAAAAAACCAAATACAACACGAATGCGGCGCTGCTCTCTCTATTTTTTCCGCTCTCACCTTTTGGCTTTAGTATTATTGTTGTTTCGCCTTCCACCATATTCGCTTTCCCATTCGCATATGTAAGAGTTGTTGTGCTTTCCTATGTACAGCTGTTTGTGCCGCTGGAACTCTAAATAGAAATCGAACAACTAACAAACACACCAACTGGTCCCAAAAACGAGTTCTTTACATTTTGTTTTACTTCTCTGGAAAATCTTTAAAAATCTTGAAAATCGTGTTTTAATATAAAATGAAAGAAATTTGTAAGAAACACATGTTTTTGTTAAGTTCTAGCATTAAACTTACAGTGTTCTTTTTAAAGAAGCTGCACTGTACCTTTAGGCGG

 

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