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CRIMIC CR00354

Gene sax

CG1891

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:7807213..7807233	43E18	FBti0199358	78897

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAAATATTGGAGGCTCTTTGCGAGTCG	CTCACCTATATATCTCTGTCTCCCGCT

Images

Construct Design Sequences

sgRNA sequence	TAGCTCCCGCTTCCGCCCAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCAGTGGACATGAAAACCC	AGATATAATCACCTGGGATGCG	TTGTAGCTCCCGCTTCCG	GGCCCGTTGATCTTCAAGGA

 

Amplicon 5'

Amplicon 3'

CGCAGTGGACATGAAAACCCTAACTAAGCAAATGTTTTGTTATATTCCATGACACCGGGTAGGTAAATCCGTTAATTTCACTCAGGAAAACAACTTAATGGCACGAAAACTCCTCCACTGAAGATCTCCTGCTTCTCCACTTACCTAAATTGAACACAATTATGCTTTGTGAACACAACGGCACTGAATAAAATAATTGTCTGTTAAATGGGCTCCTTTGCCAAAGACTCTTTGATGGGAGTTCGAGTTTGACGTTCTAGAAACTTTTCCGCTGACCGGCTGCTCGTGTTGGTGTGCGTGTTGTGCTTTGGTATTTTTACGCAGGGGATTTGGGAAATTTGAAGACCCGGTATTTTATGTGAAATATGTTATGTGCAGATGTGGGCCCTGGTCACACTGCCCGCTAGAAGTATATCGATAGGTTCCGATAGGCTCGGACAAATAACATTTCTAGCAATGGAGTCCAATATATATTTAGTTTTTCTATTGACGTTTCTGCTGTACAATAATGCCAGAGGTGGGCATCAATTAGTGTTAATCAATTACCAAGTGCTTGGTGAAGAGAGTGCGCAAGAGAAATTTCGATTTTATGGGGTTTTAGAGCCACATATAATGCCTTGAAAAGTCACTGAGTGTTTTGGGTTTGTTTTCATATGCTGCGGTTCTGGCCAATTGCGTTGCGTATTTGGCCAGGCCAAGGCGAAAAAAGTGCCCTCGGAATGCTGATTGGTGAAAGAAGCACCTGATGGATCCCACAGAATGTATTATATAAACGGATGTCGTGGGCGTTTGCAGCATAAGTGCATTTTCTCAATGATTTACCCACGCACACCACCGTTCTGTGTGTGTATACAGAACTTGCTGGGGCGCCTGTGTGCATGTGTGTGTGTGTGTTAGTGCTGGTGGCGTTTGTGTTGGTTGGGCCGTCCATGTGTGTACATATGTATATGCTATGTGCATATGGTTTAATGTGCATTTGTTATGTTTCAGCCGAAATCAGCGATCATCTGCGGGAGGACATACCGGACTTAGACATGGAGCTCTCCTCCGCCTCATCCGCCCATCTCAATGGCAAGGAGCTACCGGCCGTGCCACAGAACTCGGTGCAGACGCATCCCAGGTGATTATATCT

TTGTAGCTCCCGCTTCCGCCCAAAGGCTTTGTATTGCTAATCTCATTTCCCTCCTAATTGCCATGCTGCTAATCCTTAATTAGGAGACACATTAAGAGTAAGCGCCGCATGAAGAGAAGGTAAAACCTACACGACTCATGAACTAAAACATCATAAACTGGTTAACTTTTAGTTAAAATTTTGAAAATATTCATAATTTTACTTAAAAAACCCCATTTTTTTTAAATATTTTATAGTATATTATACTTTTATACCCAAATGTTAACAATTAAAAGCTGCCCGTTTGCGTAACCGATTCTTCTTCAATCTTATTAAACTCTCTCTTTTTTTTATCAGATACAAATGCTACTCCTGCGAGCCGCCCTGTCGGGATCCCTATGAGTTTACCCACACCTGCCAGAATGCGATTCAGGTGAATACTCCACCTTAAACTTGATTAAAACTTAAATGTAACTTAATTTCCGAACTTCAACAGTGCTGGAAGTCGCGCACACGCGATGCCGATGGTCAGGTGCAGGAGTCACGCGGCTGCAGCACCTCGCCGGATCAACTGCCCATGATCTGCAGCCAGAACTCCTTGAAGATCAACGGGCC

 

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