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CRIMIC CR00359

Gene CG2852

CG2852

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:22645800..22645804	58F3	FBti0199359	78898

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTGAAGTCTAAGGATTGGCAGACATATGC	CAATTTCTCTTCGCTAGCCTCAGTCTG

Images

Construct Design Sequences

sgRNA sequence	GCACTTTGTAGGTTATAGCGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTTTAGTCGGTGGCATCGG	TGAAGCAATTACGTGGCGTT	TGAGAGCCCCGCTATAACCT	GCTGCATGGAACGATGCATT

 

Amplicon 5'

Amplicon 3'

ACTTTAGTCGGTGGCATCGGCCTTGGCCACGGAGAAGGCCTCCGAAACGGGCAGGGTGCCGCTGTTGGCGATGACCACATCCTTGACGGGACGGTCGCGGGCATCAGTGGCCGAGTTCTCGATCTGGCGCACCACATTCATGCCCGACAGGATCTTGCCGAAGACGACGTGGCGTCCATCCAGCCAGCTGGTCTGCTTGGTGGTGATGAAGAACTGCGATCCGTTGGTGTCCTTGCCAGCGTTGGCCATGCTCAGCCAGCCGGCGCCATAGTGCTTCAGCTTGAAGTTCTCATCCTCGAAGCGCTCGCCGTAGATGGAGCGACCGCCGGTGCCGTCGCCCTTGGTGAAGTCACCGCCCTGGATCATGAAGTCCTTGATGATGCGGTGGAACTTGCTGCCCTTGTAGCTGCGGGGTATAAGAGCGTTGCATGATGGTCAGTTTTGTCAGCATGGGAATTTAAATTTAAAAAAAAACTGGGTTTAGAACTCGAAACCTAACCAAAAAAGAACATGCATACTTATAAATTCAGAGAAGAAAAAAGAAATACCCAAGAACTGCACTTTTATAGTATGGTAGACTTAATAACAGTTGGCGTTCTTTGTTAACCCATTAGTGCCGCTTTGTCAACTGATGTATGCGATTGGCCGCTCAAACGCCACGTAATTGCTTCA

TGAGAGCCCCGCTATAACCTACAAAGTGCCGCTGATTAGCGGCATCTTTTGCTGGCCTAGCGGTACCTGGCACCCTACTATGTTGGGGTATTTTCGGTACTCAAGTACCCTACTCACCCCTCGCCCTGCGGCTTCAGCGCCAGCTCCTTGAAGTTCTCCACCGTCTTGGGCACCGTCTTGCCGAACAGACCGATCTCGATGCGACCAGCGGGCTCGCCGCCAATGGTGATGTCAAAGAAAACCTACGCAATCGGAAGCGGTTTATTATTAACAAGTCATCTCACTTGATTTATGGCGCAGCAAATGTGTGTGTGTGTTTGTGTGTGTATGTACGTGGTGTATGCAGAATGTAAGGACGAAAGTCCAAGGAGCACGCACCTTCTCGGTCACTTTGGGACCCTTGCTATCGTCGGCAACAACGACGCCGGCCACCAGGGCTACCACGAAAACGGATAAGAACAGCTTCATCGTTCTAAATATTCACAGCGCTTCAGTTTATTCCTCAAGCGACGTCGCCAATGGATATTTCACGACTGAATTTTCGGTTTCTGTTAGTGATGTAAGAAAACTTTGTTGCATCGCCAATCGATGTTTTCCATCGTTTTTTATGTTTTTGTCGAAATATCGATGCTTAAGTGTGACCATTCGATGACGATGTCTGATGATCACACATTCGGGCGGAAAATTCAAATTCAAATAGTTAATACGCCGAAAGCATAAGTTAAGGTTTTCGTACGAAGGAACAAAGTAAGTTCGAAGAGTTGACTTTATTGGTATAGTTTTTAATATTTTAGGGATTAGAAGTCCAGTTTGCATTAGCACGTAGCATTCTCTCTTGTTTTATTGACGGTTCTGTACACAAAAAGTAAGTAAAAAAATATGCTTTTATTTATTGGTGTACTGCTATTTGCGTCTGAGATTTGTCCATATCCTTGGCTGCCTTCTCTGCTCTCTTCTTCTCCCTGCTGGCCAGAACTTTCTCCCTGGATTTCTGGGCCCTTTCAGCGCAATCCTTGGGTCGTGTCTTCAGGGTCATATCATCCACGGCCGCCTGTTTACGGGCTGATCTCGAAATGCATCGTTCCATGCAGC

 

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