• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00361

Gene Mccc2

CG3267

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:6747095..6747097	42C8	FBti0199569	79243

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CTACTTACCTTCTAGAGCGACCTGCA	GCTTGCGTTGATCTTCGGTGATCTGA

Images

Construct Design Sequences

sgRNA sequence	GAACAGACAGACTGGGGGCACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTATCAGTTCGGCCACCCAC	CGGCTAGATCGGATCGGC	GCCCCCAGTCTGTCTGTTC	GCAAATCCGCACACCAAAGT

 

Amplicon 5'

Amplicon 3'

TTATCAGTTCGGCCACCCACAACTGACGTAACTTTCGCCTCCACTCGCGTGGTAATTTTCGCATTTAGCTCCCTTAAAATCCCACCCCCCACAAAATGATCCGGCTTAATTGGCTATTCAGGAGCTCCTCGGTGCTGCTGCGCAGCCAGGTGCGACTGCTCCATGTGGGAGATGCCAATGTTTTGCATAGCGAGGTAGACAAGCAGTCCGCCGAGTACAAGGTGCATATAAACAGGCTGTATTTATGTTTACATACCAGCTAAAGTCCAAGCTTTTTTAGGAAAATGCCAGAGAGATGGCCAGTTTGGTTGGAGATCTGCGGAACTTCACCAGCCAGGTGCTCAAGGGAGGCGGACAAAAGGCCATCGAGCGACACACATCGCGGGGTAAACTCTTAGCCAGGGAGCGCATCAACCTGCTCCTGGATAAAGGATCGCCCTTCCTAGAACTCAGTGCTTTGGCCGGACACGAGTTATATGGCGAAGAGGTGGTCAACTCCGGGGGAATCGTCACCGGAGTGGGACGCGTTTGCGGGTAGGTCTGAAAAATCCAAGTTGATCGAAGTTCCACATCAGAACTTTTAGCGCTTGCATAGCACATTCAGTCTGCAGAAGACAAGGTTACGCGTCATTAGGGTTAATTAGGTCGTAGATTATATTTAGATTATAATTATAGATCGGCATAAATAGCCGATCCGATCTAGCCG

GCCCCCAGTCTGTCTGTTCGTATAAACGTAACGATCTCCGGAACTATTCAAGATATTTTGTGAGTCTCATAGTGAAGGTGGAAAATAAATAAATTGTGTACGAAATTTTTTAAATTATCAAAAATAATATGCAATTTTCATTACAGAACTGAGTGTTTGGTGGTGGCCAACGATGCCACTGTGAAGGGTGGAAGCTACTATCCCATTACCGTTAAGAAGCATTTGCGCGCTCAGGAGATTGCCCAAGAAAACCGCTTGCCCTGCATTTACCTTGTGGACTCGGGTGGCGCCAACCTTCCGCGCCAGGCGGATGTCTTTCCAGACAAACTGCACTTCGGACGCATCTTCTACAACCAGGCAAACATGTCGGCCCAGGGAATTCCTCAGATTGCTGTAGTTATGGGCAGCTGCACGGCCGGAGGAGCTTACGTACCGGCAATGGCCGATGAGAGCATCATTGTTAAGAAGCAGGGAACTATATTTCTTGCTGGGCCGCCGCTGGTGAAGGCGGCCACTGGAGAGGAAGTGTCTGCGGAAGATTTGGGTGGAGCAGACTTGCACTGCAAGACTTCTGGGGTCACCGACCACTATGCCTTAGACGACGAACACGCCTTGTATCTGGCCCGCCAGATTGTGAGCAACTTAAACTTGTCCGCCACTAATTCGTACAACGATCAGCTGATGCACTCCAGTCAAGTCAATTTCCAGACTGCTACTCCGCCGTCTGCCGTAGAAGAACCGCGCTACGACGCGGAAGAGCTGTACGGCATCGTAGGTCCCAATCTTACCAAAAGCTTTGACGTTCGCGAGGTGATTGCGCGCATCGTCGACGGAAGTCGCTTTACGGAGTTTAAGAAGCTATATGGTGAGACTTTGGTGTGCGGATTTGC

 

---

Search Again