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CRIMIC CR00362

Gene pic

CG7769

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:13053097..13053097	87D11	FBti0200547	83172

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	AATTTGAAGGCGTCCCAGAGCTGCAA	GGCAGCGACAGGATAACAGCAGATATA

Images

Construct Design Sequences

sgRNA sequence	AAACAGGGTAAAACATGATAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTGTTTGCTTAGCACCGGC	AAGGCGCCGATGTCATGC	TCGGCGCCTTATCATGTTTT	TCCGGGAATCTCGGTCTCTT

 

Amplicon 5'

Amplicon 3'

TTTGTTTGCTTAGCACCGGCTTCTTTGGTATGCTCCTCAGGCAACTTCACCTCGCCCACGGATCTCAATTTGATCATTGCCCGGAACAATCAGGTGGAAATCGATCTGGTCACGCCGGAGGGACTGCGCCCCCTAAAAGAGATCAACATAAATGGCACCATTGCCGTGATGCGCCACTTCCGACCGCCCGACAGCAACAAGGATTTGCTCTTCATACTGACGCGTCGTTACAACGTCATGATCCTCGAGGCCCGTATGGTCAACGACGTCATCACGGTGGTGACCAAGGCCAATGGTAATGTTTCCGACTCGGTGGGCATCCCCTCCGAAGGAGGAGTTATTGCGGCCATCGATCCCAAGGCTAGGGTCATCGGAATGTGCCTTTACCAGGGGCTGTTCACCATCATTCCGATGGACAAGGATGCTAGCGAGCTGAAAGCCACAAATCTTAGGTAATTCCGGTGAATTCTATAGTAGTTTGACAAATACGTGTGAGCATGACATCGGCGCCTT

TCGGCGCCTTATCATGTTTTACCCTGTTTTGTATTTTTAATGAATCATTTTCATTTTTTCATTCACTACTAGTTACTAGTAAGTAACTAGTAAATGTTTTATGATTGAAGCTGTATTAAGCTCCAAAATAATGTATCCCTTCACCCTTCAGAATGGATGAGCTAAACGTGTACGATGTTGAGTTTCTGCACGGCTGCTTGAATCCCACAGTTATCGTGATCCACAAGGACAGCGATGGCCGGCACGTAAAGTCGCACGAGATTAATCTGCGGGATAAGGAGTTTATGAAAATCGCATGGAAGCAGGACAACGTGGAAACAGAGGCCACAATGCTGATCCCGGTGCCGTCGCCCATCGGTGGTGTTATCGTCATTGGTCGGGAATCGATTGTATACCATGACGGAAGCAACTATCACGCTGTGGCGCCGCTCACTTTTCGCCAAAGCACCATCAACTGTTACGCCCGTGTGAGTAGCAATGGATTGCGCTATCTGCTGGGTAACATGGATGGTCAGCTGTATATGCTGTTCCTTGGGACCGCCGAAACTAGCAAGGGAGTGACTGTGAAGGACATAAAAGTGGAGCAACTAGGCGAGATCTCTATACCAGAGTGCATTACATACCTAGACAATGGTTTCCTTTACATTGGAGCACGTCATGGAGACTCGCAGTTGGTGCGCTTGAATTCGGAGGCCATCGACGGGTCATATGTCGTGCCTGTAGAGAATTTCACCAATCTAGCTCCCATTCTGGACATTGCTGTGGTCGATCTGGATCGCCAGGGACAGGGCCAAATCATCACATGCTCGGGAAGCTTTAAGGATGGATCTCTACGCATAATTCGCATAGGCATCGGCATTCAGGAGCATGCCTGCATCGATCTCCCCGGCATTAAGGGCATGTGGTCTCTAAAGGTGGGAGTCGATGAGAGTCCGTATGAGAACACTTTGGTGCTGGCTTTTGTGGGACACACAAGGATTCTCACGCTTTCTGGTGAAGAGGTGGAAGAGACCGAGATTCCCGGA

 

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