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CRIMIC CR00365

Gene Wnt6

CG4969

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:7338257..7338264	27F2	FBti0199570	79244

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	GCGGATCTGCGCAATTTGCATTTTGCAA	AAGTGAAGTGCTGTTGTGGCTGGTC

Images

Construct Design Sequences

sgRNA sequence	GGTCAAGTTGGAGGACGGTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGTCAGTGCCCCTCATCTG	CGTCCTCCAACTTGACCCAT	CCCATAACCAGGTAGAGGGC	ATTTCGTCCCGAGCTTGAGG

 

Amplicon 5'

Amplicon 3'

CAGTCAGTGCCCCTCATCTGGTGATTTTTCTTCTATTTTTTTTTGTTTTTTTTTGGCTCTCTCAGCATGTGAAATGGTAATTAATGCCAGGCGCGGCATTAATGGGTTAAGGCAAACAGGGGGTGGTGGGTGGCCAGTGGCCCAGTGGCCACTTAGTCTCAAGCTGTGCTGGCTCCACAGTCGGTGGTTGCATCAACAACATTGCTCATACGCCCCGTGCACCACGAGATTAACGCTGCCGCGAATTTGGCACACTTCATGAGCCAGCAGCAACAAGCACCCCCAACAAAAAAGCAAAAACAACACAATAATTATGTTCAACTAACGAGCAGACAAAAGTCGGCCAATTGCCAAAATCCAATGCAGATGAGTTGATTATGAGCAGCAAAAAAGCGTCTCTGACACAATTCGCTCGACATTAACGAGAATACCTGTGCCCGTCCGTGTCTCTAGAGAAAGTTTCACGTTCAAGTTTCTTTCCCCAACCTTAAGTCCCAAAAGTAGCTAGTTACCGTACACTAGATCGCTGCAAATCTCTTAGTCAAGAGTCATGCCATTTGTTTATTTTGTTAATTTGTCGCTTTCCCCTTGTTACATTGACGAATGTGTCTGTCTTCGGATTGTCTGCCAGATGCATCATAATTAAATTGGATGACGAACCAAAGAACGCGGACAGACATGGGTCAAGTTGGAGGACG

CCCATAACCAGGTAGAGGGCTTGGCCATGATGTCTGTCAAGCATTCTGCGCCAAGTGGCCCAAGAATATGTGCTCCTCGTCATCTCGAAGGTGTGATGATGCCCTGGGACCAGCGGCTTAAGAAAGTACGGAAGCTGATTAATACATACAAAAAATATATAAAAATTTGTGTTTTGTCAGAATTTTATTACATTATAAAAGGACCATACTGCACATTAAGTATTCTATTTACCTTGCAATTTCCACATCATAACAAAATACTTTTTGATCTCTAATGGCGCTGGCATCCTAAGCTCCAAGGAATCCTTGTCCAATGGCCGGTGCCCAATTCCTCTTTCCCACAGCCTCAATCATCGTGATTAAGCACAGTTTAGACATCCTTGACATGAACATGACCATGTCCGTGCGCCGATGATCGTTCCCTAGGCCCAGCTACGCCTATTTGAGCCGTAATTAAGTTAAACGTTGTCGCAATTTGCGCACCTCAAAGTCACCAAGCCCAGAGATTGATGTCGCCTTTGTTGGCCCAGAGAGTTAAGCCAGGTTAAGGCACTTAGGGCTAAAGTTGAAGTCGTGCCCTGCCCACTGGAACCAACGAAGGAGTCTTGTTAAAGGAATGACATTGCCTGGCATCGCAGAAGACACTCATAGTAAATATGCCTGAATTATTAGCCAGCGTTTTTGCTGCGCCAATTGGACATGGACATGTCTGGGAAACAGTAAGAAAGGCTAGCTTAACAGATATGAGCACTAACTTAAAATATGTTTAGAATATATAAGGGTAAACTTAGTGCTTGAATAAAAATAGAAATAATAGCAATGTGGCATTATAATGGTTTTATTCCCTCAAGCTCGGGACGAAAT

 

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