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CRIMIC CR00368

Gene stan

CG11895

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:10717441..10717445	47B7	FBti0199361	78900

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATGATGATGAATCCCGGACAGCTAG	CGAATCATTTTATGCACACCCTAGCTACG

Images

Construct Design Sequences

sgRNA sequence	CGAGACTTCTGCGACACACTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATGCCGCGTACTTGAGTGA	GGGTTGGACAATCTTGCTGC	GTGTCGCAGAAGTCTCGAAT	TTCAAACGTTCCCAGGACCA

 

Amplicon 5'

Amplicon 3'

GATGCCGCGTACTTGAGTGACAACATATACGACAAGCAGCGAACGCTGGGCAGTGGCTACTTGGGCGCCAAGTCGGAGTCGCCTTACCTGTCCAAAGATCGCATAACACCCGATATCTATGGATCCAGGGATGGTCATTATAGCCTCAAGAGACAGCCTGCCTATGCAACTGATTCCCTGCACTCGGTTCACTCGCTCCTAAAGAACGATTACCACCAGCAGCAACAGCAGCAGCAGCAACACCATTTGCAAGACAGATTGTCCGAGGGATCGGATAAGAATGGCTACCACTTTCCCTACACCGCCGAAGAGGATCACCTGCCCGCCAGGAAGCTGAGCCACACCCAACCGCCTTCGCTTCACGGATCCCAGCTGATGCAGCCACCTGGAGTGGGCCTGGTCAACGATGTGAACAATCCGGGCTTGATGGGAAGGCACACCCTAAATGGAGGATCCCGACACAGTTCCAGAGCGTCCTCGCCGCCCTCCACCATGGTGGCTCCCATGCAGCCACTGGGCCCACTGACAAGCATCACGGATACCGAGTAAGTTGGCCAAAAGGTCTGCAGCAAGATTGTCCAACCC

GTGTCGCAGAAGTCTCGAATTTTGTACCAAATTCCCTGGAAAATCGTATGGCACCATTGGTAACATTCGGTTTTGGGAGATTTCTGTGACATGCCTGCTCCTGTTGGCTTCGGTTTAGGTTTTGGTTTTGGTTTCAGTTTTGTTGTGGTTCTGGTGACCAGTTAATGCTTCTTGTGCTCCTTGTGCAGCCCATCCTGTCTCCTGATTTGAGTTTTATGTTGGCCTAGATGATTAGTTTTGGTTCGGGAGTTCCTGTTCGTTCTCCGTTTTTGAATCCAGCAATTAATCTGTGGTCCTTTGTTTCTGATTCTCTTTTCTTTATGGCATTCGAATCAACAACTACAATGACATAAACCCTTTTGTGCCATTCGACTTTTCCCACAACAATGAAAACTGAAAACAATGCAAATGAATATGGTACAAACAACACACACTCACTGCACGCCTACACACACATGCACACTCGTACCCACATATCATATACATCTTTCTATACGTATACATGTCCATATCCTTTCAGCTGGTCCTGGGAACGTTTGAA

 

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