• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00369

Gene tio

CG12630

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:22020146..22020150	40D3	FBti0199571	79245

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	short band	AACCTTGATTTAAGCGGCAGCGACGT	ATGCTGAGTCTCCTTCATGTGGGCA

Images

Construct Design Sequences

sgRNA sequence	ATTCGATACGGGGTTTCCCGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CATGTCGCCTTTGAACGGTG	AAACCCCGTATCGAATCGCT	GAGGAGGTTGGGCAGTTCC	CTTCCTGATCGTCGTCTCCG

 

Amplicon 5'

Amplicon 3'

CATGTCGCCTTTGAACGGTGGGTGGTGCAGCAGTAACGGTACATCGCTCGACCTACGACTAAATTGCGAGTGCAGAAAATAGTGTTCACGGAATTAACAGACCTCGAAAATCCAAGTGTAGGCGAATAAATAAACGAAATTATCGTGGAAAGCCATCAATAAATCACCAAATTAATAGCCCTGACGAGCACCCACGGCATACGCACAGCTGTTGTGTGTGCAAAGTGAGAAGAGGAAGCTAAAGGAAGTGAAATTGGCGGCAGTTTGACCGCAACAGCAATAAAATACGTCTGCAACCCACAAACGCACGCTCACATACACCCGTTCGTCATCGAATCGTCGTGGTCCTCCGCTTCCCAGTCCTCGCCCGCCCCTCTTCGACACACGAACCATTGTGACATTTGATTTATATGACGTGTGTGTTTTTGATAGAAGTTCAACACGAAACTGTCACACAAACACACGTATGCTCGCCGAGGAATTTATTCAGTGAACGAAAATAAAATCAGAGCTCCGTACGCACTTGGCCGCAGTGAAACGAATCCGCCACCATCGATGAGCAGGCGGACGAAAGTCGGAAGTATCGCCAGGCAGTCCAGTAATCAAACCTTTGGAGAGCGAGCCCGGATACCCAGTTTTCTACAAAATGATGCTGCACGAGGCCGTCATGCTGGAAATCTATCGCCAGGCGCTGAGTGCAAGGTGAGCGCTTCCTCCGATTTGCTGTTCCACGCCAATAGCGATTCGATACGGGGTTT

GAGGAGGTTGGGCAGTTCCCTTTGGAGATGCAGTGCTTTATTTCGATTATTTAGTCGAAAGCTGCATAAAGTTAATTGGGCATAAATAAAAGATATAACTAAAACAACGCAGGCGGCATTTTGAATCGAGTTTGTTCACCCTGCTGAAAAGTTTGTTGTGAATGCAACTTCTTGCTGTGCCGATTGATTAGTGTGAAGTTTACTCCATTTGGCACGGTTTGCGTTGATAAACGAAAAAATGCCGCTTAGAAAAGATCTTTCCTAGGTATTAAAATTTTTATTTTCTCAGCGCGATTAATGTGGCTCTTCGTATGAACAAATAATCAATTAGTCATTACAATATAATTATTCTGCTCCACAATGGAATGCTTGCTTTCCTAGTGATACCAAATTGACAGAAGCACTGTTGCTGTTTTAATGTCCGATATTTAAAGAGCTTAAAGTACGAAATTTTAAATAGAATATATATATATACCTTTTTTTCGCATGTAATTGTGACTCAATTCTAACTTCCATCACCTTCGAATTTTCATTTTAGTGAGTTAACTAGTCCGCGATGTCAGTCTCGCGATTCCAACACCTCAGCTGGAGCGGGTGCTGGAATGGCCGATGTCCGATGTCCATCGAATGAGTCGCATTGTTCGGCGAACGACCGTCTGACCCCAGCTGCAACGCCCACTCTCACGCCTACTGAAGCAACCATCTCCCCAAACTCGGTGGGACTTCCCCTGACGGCGACCCTTCCTCCTGCAGCTGCAGTTGCTCTGCTACCGCCCCAATCCGCCGCCATGGCCGCTTACCTAGCCGCTGCCCAACAAAATCACCTTCTGCTAACAAATCCATTGGCGGCAGCAGCTTCACTGGTCCAACATGCAACGCAGCAGGCAGTGGTGGAGGGGGAGGTGGAGTCGCCGGCCCTCGATTTTAGCAGAAAGCGGCCTAAGAGCCACGGAGACGACGATCAGGAAG

 

---

Search Again