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CRIMIC CR00375

Gene Hs3st-A

CG33147

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:18747630..18747641	55E12	FBti0199572	79246

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	short band	CAATACCACCAGAGACAGCAGCAACA	TGTAGGTAGAGCATCTCCAGCAGC

Images

Construct Design Sequences

sgRNA sequence	CGGGAATGATGAGGGTGAGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCAGAGGATTGGAGACAGG	GTCCGCTATGTCGGGCATTA	CCCTCATCATTCCCGTACCG	CATGGGATGGCTGAGCTGAT

 

Amplicon 5'

Amplicon 3'

GGCAGAGGATTGGAGACAGGGACAAATGAAGCGTTACAAATGCTTAGTACTGCGAAATGGAACTTATTCCAGTTTTGTCAGGATTAGAGTGCTTGAGCCCGTCTTTAAAGCTTTGCGTTCGGCGGGGATTTGGGCTTAAAGGCTGCCTGTCTGCCAACCTAGAAAGTGTGTGACAACGACAGAACGAAAAGGAGCAGAAACAGCTAGAGAGGGAGGACTCCTTTCTGGGTGGCGCTGCGTTTATGCAGATCGCAGTCAAGTGGCGGGCCGCCTTGTGGAGCGCGAAACGAGGCCAGTCGCAGCCGGGCTGGTACACTCAGAGAAATACATAAGTGAAAAGATATGGGCTGGTAGAAGTTGCAATCATATTGTTAAGATCTCAGATTAAGTTCTCAGAACGATACTTATTATTCTTTAAAAACATTATTTTTTTAAATAATAGCAGATATTTCTAGGTAGGATTTTATAATTGTTTTTCTTTCCGTGTTGTTACTGCAGCTGCGGGCTTAAAACGGGTTAAGAAAATGTAAAAAAAAAAAAAAAACAGCTGGGAATATAAAAAGAACTTAAAGCGTGTCGACAACTGGCAGAAAAAGGAATGGGGGTAGAGGGGATGCAGGGTATCCAGGGCGAAAATATAGAAATGGGGGGTATGTAGAATGGCAGGAAGTCGGTCGGTCTTGCTCTGAGGTTGCTCCGTGCGCCGCAAACGGCGCCGTCGAGTGAAGTGCCTCGACTCCTTGCGGGCCTCTAGCCCAGTGTGTCGTTACTTTTAACGAGGTGTCGCATGCGAAGTGGATGAGGCACTGGCAGCCACTGGCGGCGGCAGGGAGGAAACTCCAGGGGCGAAGCCACCAGTTGCCACACGTTTCCCAGCCATGCCCTGCCCTGCCCCACCAGTACCACCACATCACTTTGCTATGGCTATGCCTTTGCCACCACGCCCCTACTCTGTATATGAAATTGCACTTAGCCGCAGACTGGCAGCAGCTCCTTCTGTCACCTGCTAATGCCCGACATAGCGGAC

CCCTCATCATTCCCGTACCGTGCAGCTGCGGCATGCTGCAAATGAAGTCATCCATTAGGCCACGTTACGTATACGCCTAGTGTGCTGCAATGTGGTGCTCATAATAATGCTGCCAGTGTTTGTTGCGGTCTAAGCGGAAACGTTTATAATGGAATTCGCTTAAGCGCCGCCGGACGTGAGCGTTCAGGTGGTGGCTGAAAATGGAGGACTGCGTCCAGAGTGTCGTCTTGCTAATTTAGCTAAAGGCAACCGGAAAACGGGAGGAACAGTAGTGGGTTGAACAGAAGTGCTGGAAAACCGCCAGCCTTAATTACTCAGGACAGCCGGCAGGGCAATCTAATTAGCTGAAACCAAAACCAAATCCTAATCGATGTCCTCTCTATCTCTCTCTCTCTCTCTCTCTCTTATAGATGGCTACCGAAATCGGCAATGCGGCCGGCGGGTCCTGATACCTCGCCCACGGACTGCATCCTGGTGGTGGGCGTTTCCCGTCCAAAATTGGCCGTCGTCTTCCTTACGGTCATGTTGATCTCTCTGTTCCTCACCTTCCATGTCCTGTACGATAGTGCCGTATACAATATCCAAGCGGCCCAGGCTGTCCACGAAAGGCATCGTCTATCTATGACCGCCTCCGCATCGGCTCTGGCATCCTCCTCCTCATCGTCGTCCAATTCCGGCTCCTCCTTCTCCTCCGCCGGTGGCGCCTCCAATCATCTGCCCGTGTTCGTAAAGCCCGTGCCCAGCTCCAATCAGCTCAGCCATCCCATG

 

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