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CRIMIC CR00381

Gene CG12512

CG12512

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:5341937..5341953	25D6	FBti0199574	79248

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	no band	ACCATCCATACAAATCCCCTCGCAAG	GGCAGAATGCTCTTCTGCATTTTGGC

Images

Construct Design Sequences

sgRNA sequence	ATGCCCATCCTTATAGATGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGTGACAGGAGACAATCGC	GGGGCCAAGGTCACAGTG	TGGGCATCCTTAAAGGTCCTG	TCACGCCTCTGCAAAAGCTA

 

Amplicon 5'

Amplicon 3'

TCGTGACAGGAGACAATCGCCTCCACATCCCCAAAATTCGAAGCAGATAGCTCCAACTGCTGGCCAATGGTGCGATATACCAAGGGATCCTTACCAATATGATGCTTATGGCTGATCAAGCTGGGCATCGAGGTGGAAATGCTGCAAAAGAAGAAGGTAATGCCATTTATTAATATAAGTAAGCTAAGCTGGTAGGACGAATAACTAATAAGATAATATTCTTTATATCAGAATTCTTATAATCATTTGTTAAAAACGCATGCCTGTGATTATTTTTGAAAGATTCTACGTGTCACAAAGCTATATTTTACCATGGGCGGTTTGCGTTTTCGCTCAAGGGGTTTCAGATTTAGTTAATGTCGAAGCTTTTTCAGATGAAAAAGCTTAACACTAATATCGATGCAAGTCATAGGAGTTTACATTTGCAAACATATATGTTTTATCACTCGATAGTTCTGCTGACAGATTATACTTTCGAATTGTAAGCGTAACTTTAGCCGCTGCCAAGTGGATAAGAGGGGGTGCTTCAGAAGTCGCACAATAATATTCATTTAGTAGTGGCCAATTGTAAACTATGCAATTTATATGCTTATACATATTTGTAAATGTAAACACTTGTAATGTGAAAACACTGGCTATCTGTTGCGGCGCTATGATAATAGATCTTAGACTTGCGCTTTTGGCTTCGGTGCTGGTCATATACATATTATCATCAAGCGAGTGGAAAGTTCTGCTCACGATTTGCTTATCTTTTGAAATTCATCTAAATTGATACCCGATAATTAAAACGACCTGTTGCTGGTCTAAATTTGAGCACCACTCACCCAGCCACGCCCCCTTTAAGTTTGGTTCTAAATTTGAGGACTTTTACTTAGCCCTAATGACTACCAATTACTGTGGGCAACTGTACCTTCTATCTGGAATCAGAAATATTACGCAACGTGGCGTAGGGGGATGTGGTATATGTGAGGGGCATAGGGGGGGTTGTATATAAAACTGGGCACCCCAAACAAAACTGCGCAATAAGATGGTGGAAACTTCCCAACCCTTCTTCACCAGCAGACACTGTGACCTTGGCCCC

TGGGCATCCTTAAAGGTCCTGATCCTGCCTACCTGCGCATCGAGTTGAAGGCACATTTCTGCCTGAGCATGTATCGGGAGATGGTCTGCAGCTTGCTGTTCATCTTGGCACTTATCACAGTTAATTGTTATAAACAGCTGTTTTGTTTTATTATATTTTCATAATATGCACAGGTACACTTTTCACTAAAAACGACTATTAGTTTTCAATTCAGTTCCGCTCGGTTTCTATTGTATGGCAATCTAGGAACGACCGCAGTCCGTCAAGTTCTGAAACAAACTGAGTGTGGGGGGGTCTTCTAATCGCCGTATGGTTCTGCCAGCTTTGCTGCCTCTGTCGACGCCTTAAGTTTTTTTTTATATTTCATTAGTTTTTTTTTTCTTTATAGCTAGACAAGATCGTTGCCGGCGTTTGTTTTGCTTTCGGGTTTTAACGCTTTTATTTTGCTTGCTTTGGCAGCACGTGTGTGACTGTGAGTTAGTATTGTGTGTGTGTTGTGCTTTTAGTTCTATTATGTTTGCTTAATCTGTAGCTTTTGCAGAGGCGTGA

 

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