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CRIMIC CR00389

Gene Cyp305a1

CG8733

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:19812677..19812691	76C6	FBti0199575	79249

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TTGGAATTGTCGAGAAGAGCGGTCG	GTGCTCCTCGATGGTGTCCATAAAGA

Images

Construct Design Sequences

sgRNA sequence	ATCAGCCCGGCCCTTGTTACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGCAGACGCAGAAAGAAGT	CAAGGGCCGGGCTGATTTAT	CAGACCAGTTAGGCCAGGTG	CTTGGACGTCGGTCTCGTTT

 

Amplicon 5'

Amplicon 3'

TCGCAGACGCAGAAAGAAGTTATCTGGTCGCCCCTCGAACACCTCCTGCAGCTGCACTTCCTTCACCATCTCGTGGCCAAGGGCAACCACCACATATTCGCGGCCCAGCTTCAGACCCACCAGGTCGCTCCGGAAGTCTTTTGCCCAGCGCTCGAACAGGATGTGCTGGCCGCCAACAGCACTAGCTTCCTTACGGAACTGGTAATATAATAAAGGAACGAAGGATTAATAACCCTAGTATATTTAATGATTTTGAGAATATATAGGTTTTTGAGATCTTGATGAACATATTCAGCTTGCCCTTTTCGACGAAACGTTATTTAAGATTTGGATTTCATTAAAGAACAATCTAGATTTTGGATGTTTCAATTATCTGTTTCGTATTCAAATAATCTCATTTTAATAGTATTTAAGAAACTTTTGTGAGCCTTACCTGTAATGTGTTTCCTAACCATGGCACAAATCGAGGTCCTGTTTTAGGAATGAAATTCAAAAATGATACTAATTTTAAATATTTGTCTTGTTAGCAATAGAATTTCATATGGAATACAACCGTCCAATTCAATCAATTCAACCTATGCATAGGTATCAAAGTGCTAATCTAATCGCCTGCAAAAGAAATCTGTTAGCAAGCAAAACTACCGAAACGAAAGTGAAAACTCTATCTTGAAATCAAACCGCTTAGTTATTAACTATCAATGCGTTATCAAAATCAATTTCATAACTTTTATATGTGGTCAAATTGAGACCGCTTTTCTTTAGGTGAATAAGATTCCCTTAGAAATTGCATACTAATTTATCACCATTTTGAACTGCAACAATTGCGAATAATAATAAAAATGTGCTTATATACAGGAATGACAGCTATTACGCCCATAATCGTAATCCTAATGCAAATCAAAAGCTGCATTGGTAAGTGGAATAAATCAGCCCGGCCCTTG

CAGACCAGTTAGGCCAGGTGTCAGAAGTATGTGACGATTTTATCAATAAGATATATTAGCATTCAAAGCGTGTGCCGTCTGGTTCCCCGCCGATTGGCCAAAGATGCCTCTGATCACCATTACGAGTTGCTAATGAGCCCACCAATTAATTGGTCGCGGGGCAGGTCGTATGTTCGCCTATATACAAATATATTTTATAATAATATGGTTATTAATGCAAAGAATAATGCTTCTCTTCTATTAAATATAATTAATTGGCTAGTTTTCAAAAGCAAACTGTACCCACGATACACAAAATTGGTATTCTACGAATTTTAAAGCAAATCCATCGTTTGTTTGTGCACCAACGCTTTAACTTCTTATCTGCATTTGGGATTCCAGGAAAAGTTTTCCAAAGTGCAGCACGAGAAGAACTCACCTGGTGGAAAGTTCTTGGGACGCCGCGCCGAGCTGATGAGTGAATAAATCACGAAACCAATTAGAATCGCGCACAGAAATATGAGGGCACTCATTTCAATATACTATTTATAAGACCACCGAAAAACGAGACCGACGTCCAAG

 

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