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CRIMIC CR00390

Gene ClC-c

CG5284

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:16091951..16091978	72D5	FBti0199576	79250

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACAGCTACATAACCGCCTACCAAACGG	ATGGCAGTGACCAGGGTAACGAAGAG

Images

Construct Design Sequences

sgRNA sequence	AATATTCGGAACCGGCAATGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACAAAGCGGTAGAGGCTAGC	GGTGACGTCGCCTTACTCAA	CGGCTCACAGCTGTACTCAT	GGGAATACCAGATCCGCAGG

 

Amplicon 5'

Amplicon 3'

ACAAAGCGGTAGAGGCTAGCTAGCTCTCCAAATTCACAAACCTTATCTTATAGCTTTTTTGCTTATTTCGTTTTTTCAGTCGAACGGCCACACAAACGGAGCCGGCGGCGACGGCGACCTAGACGGAACTTTTCGTGACCATCCGCTGGCGCAGCACACCAAACTGACCACCTCGCTGACGGCGGCGGGCACCGACGACGAGGATATGATTGACATCACACCCCGCTCCAGTCCAGGCGATGTGATCGGCGGCGGCGGAGGAGGAGGAAGCAGCAGTGGCTATCACCGGGAAACGGCCACCGAATCGGACACCGAACGAGATTTCGGACACTCTGGCGGCAACGGCGGCGGCAATCACCACAATCAGCGGACAATGCACCAGCATGTGCCAACTCACAGTGCCTTCGGCGATCCTATGGACGGTGAGTTCGGAGAGATCCATTGCGTAAAACATCCAATGTTGCTGTCTGTGGTGTTAATCAAGATCACGAACATTTGAGTAAGGCGACGTCACC

CGGCTCACAGCTGTACTCATTCGCTGGCAATTCAATTAATATGTACAATCATTAGCATACAGACATGTATTGTGGAGACACCAGTTAACTTCATGATATATGTACATACGTATATTAATACAATCACCATTTACACACGTGATGCTAATTTAGCAATATAATCACTGAGTTGTCTAAACAACCAGCTGGCATTTCTATTGGGAATTATTTATTTAAACGAAAAAGAATCAATTATAGTTGTTGGGTAAATACTATCGTATATCTGATGATAATAGCAATCAATGTTTACAATACGCTAGAACGAGTAATATCTGTAAGAACTTTATTCGAGGAATCTGTTTAAGAAAACGTAAACGTTCTTCGTCTCGATACCTGAATGAAACACGAGAAAAATACTATAAATTTTTGCGGTTATAAGGCTCTGGTTCTCTTTGGACTTTGACACCAAACCATTAGAATTAGCGTAATCACGTGTAATGCTACTACGACAACATGATTCGGAGCTTACTGTGTCAAATCCGTAATGTTTATTGTATTATTTATTATTGCTATAGGAGCACTATCCTTCTATGGTTCCTCGGACGTGCAAGATGATATTCCTGGCATTGGGCAGTACGAAGATTTTCACACCATTGATTGGCAGCGGGACATTGCCCGTGATCGGATGCGACATCGCTACATAGTCAAGAAGCGACAGGACTCCTTGTGGGATCTGATAAAGGTATAAGATCGTTTATGATTCAAAACACACCATACTTATCAATCATTTTACTTAAAGGGTTCCATTGATGCCGGATCTGGCTGGCTATGTGTTTTACTCGTCGGAATTGCCGCAGGCTGTGTGGCGGGCATGGTGGACATTGGAGCTAGTTGGATGTCGGATCTAAAGCATGGCATTTGTCCACCCGCCTTTTGGTTTAACAGGGAACAATGCTGCTATCCGGCCAAACAGTCAGTATTTGAAGAAGGCAACTGCTCGACGGTGAGTGAAACTACATTTTCTTCCTGATAAGGCACTAAAACCGCATTTATTTTCAGTGGAAAACCTGGCCAGAGATCTTTGGTTTGGATCGAAATGGCACCGGACCATATATCGTCGCTTACATCTGGTATGTGCTGTGGGCTTTGCTTTTTGCTTCGCTCAGCGCCTCCCTTGTGCGAATGTTTGCGCCCTACGCCTGCGGATCTGGTATTCCC

 

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