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CRIMIC CR00395

Gene unc-119

CG1659

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:7289387..7289387	7B1	FBti0199364	78903

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GTGCACGAAATGCCCTCGATTTAACC	GGGTAGCGGAGACGAAGCCTTATATT

Images

Construct Design Sequences

sgRNA sequence	CTGTGAGATGAGGCTTCTATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAACACAATCCGCGTGCTTC	TGTGAGATGAGGCTTCTATTGGT	ACAGGCGCAAATCGTAAACG	GGAGTCTTCCAGCGTGACTC

 

Amplicon 5'

Amplicon 3'

GAACACAATCCGCGTGCTTCAGTGTGTTGTTGTTGTGTTTGTGTGTTAGTTTCCGTTTGCAGGAGATCATATCTTGATGCTAAATACATCAAACAGGAATGGTGGGTAAAACTTTGGTGTGCTAAGGCTAAAATGGCTGTGATAATATTCCTCGATTCGATTCGATGTGTTTGTGAGTGTGATTGTTGACATTGAGTGGGTGTTTCGTGTGTGTGTGTGTGTGACAAGTCATTGATTTTGTCTCACAGCTTCTGTAGTTTCTAGTTCTTTAGTTATTAGTTTTGCTCTAGTTGCAGTTCCATTATAGTTGAGTTTCTAGACTATATTGCCGCCATCGTAGGCGTAGTCGGCTTTGTTGTGCATGACGAGTCGGTTTCCCACAAAGTAAAAGCTGTCCGAGCGCGTCTCAAAGGGGCTCGATATCATCTCAGCAACTAAAAAAAAATGGAAAACGAACGAACATATTTATGTGAATTGCATTTCATTAATAAGTCCACATCCTACAAACCGATAACAAAAAATAAAAACCATGATAGCTGCTCGCAATCTGACCAATAGAAGCCTCATCTCACA

ACAGGCGCAAATCGTAAACGACCTTGACCCACAAACCCCTTCAACTTTTGGCAGCAACTTTCAATTGCGTGTCATGCTGGCTGGGATTATGTAAAAATTGCTCACCTAGGTCGGGTGGAAGGTTAGGAAACTCGTAGATGTGCTCAACCGTATTCTTCGAAAATGGAAAGCAAAAGCCGAACTCAAAGTCAAACGTCTTTAGCAGGCGATCGCGGAAGAAGTGGCGTTCGATCATACGAAAATTATTAAGCGGCTGGCTGCCCACAGTGAATTCCACACTGCAAATAAGGGAAACTTACAAATTGAGAGGCATTTAAAACAAATGCAAATACTGTATAACCTACGTGGCTCCCACTGTTTTGAGGTTGAGAAATGCCGGTGTGAACTGATAGCGCACATAGCGTCCGGCATTTGGATCGGCAGTGTCATCCAGTGACAATTTCTCGGCAGCCGCCAGCATGGTTTCATCCGAGGACAGTCCTTCCGGATATTGTTCACTCGGCGGTTTGGCGATCTCGAAGAGCACAGCGCCGCTCTCCAGGTCGCGAATCTTGAACCGCGTGAAATCAATCTCGAACACATTTGCATTGGCGGAGCAGAGATAGTCGTCCGTGATCTTGGTCAGATGGAGCACCTCGTCGGGAGTCACGCTGGAAGACTCC

 

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