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CRIMIC CR00398

Gene spas

CG5977

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:24039410..24039420	95D9	FBti0199578	79252

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	TGTCCCATTCCGACTGCTCTCCAA	CTGATGCGCTTGAGCGAACTGTGAAA

Images

Construct Design Sequences

sgRNA sequence	CCTACTCCTGTTGACATTTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTCACGGGGTTAGGGCAAG	GACATTTGGGGCATCCATTG	AGGGTTGTCTGCCTTGATGG	TTTATGGGAGTCCGCGAACG

 

Amplicon 5'

Amplicon 3'

TTTCACGGGGTTAGGGCAAGGAGAGCAGGGGGCCGGAGTGCTGGACGCGCAGACAGCGCGGAATCGATATCGATGGTACGCACTAAAAACCAGTCGTCCTCCTCCAGCGCCAGCAGCAGCAGCACCAAGTCACCAATAAAGTCCAGCAGCGGGGCAGGATCATCCGGCGGAGGACTCGGTGGTCGCCAGTCCACTCACCGCTCGTCGAGCGCCTCAAACGTTGCTGCCGTTGTTGCCGGCGGTTCATCTGCCGCCGGTGGAGGCTCCTCATCGAATCGCCGTAGTCCGGGCAGCTCACCCGACGGCGACGACGACACCACCACCACAGATGACCTGACCCCCACAACGTGCTCCCCACGCAGCGGCCACCACCATTCGTACGGCGGCTACTCGTCCTCTGTGCACAAACAGAACCTCTACGTCGTCTCGTTCCCCATAATCTTTCTTTTCAACGTTCTGCGCTCGCTGATTTATCAGCTGTTTTGTATTTTTCGCTACCTATACGGTGCGAGCACAAAGGTGATATACCGCCCGCACCGACGCGACTGTAATATTGAAATCGTGGTGCAGAATTCCTCCAAGGAGCAACAGCAGTCGTTGAACCATCCTTCGGAGCTGAACCGTGAAGGTGACGGACAGGAACAGCAGTTATCCAATCAGCCACAGCGTTTCAGGCCCATTCAACCGTTGGAGATGGCCGCCAATCGTCCGGGAGGAGGGTATTCACCTGGTCCCGGCGATCCATTGCTGGCCAAGCAGAAGCATCACCATCGACGCGCCTTCGAGTACATCTCCAAGGCGCTCAAAATTGATGAGGAGAACGAAGGTGAGTTTCGGCGACGGGGGTGGCAGCTCCAGTTCGAGTTCGAGTGCTCGAATGCTCAATGAGTCATCGCCCGCTGTCCGCTTTTGTTGTCTGTCGAGTGTGAGAATCAATAAGGCAAAACGCGCGCACAATGGATGCCCCAAATGTC

AGGGTTGTCTGCCTTGATGGACTTTTATGGAGTGGAAGTTCTCCAAGCATGCGATTTTTAAATTCTAATGCATATAAGCGTACTCTTTGTCAAATTATTTATAAGAGGAAAGCGAGCTTGATTGAACATAATAGTTATAAAATAAACATAAAATAATATTATTCTATATATTCAGGTCACAAAGAGCTGGCAATTGAGCTCTACCGAAAAGGTATCAAGGAGCTAGAGGATGGCATAGCTGTCGATTGCTGGAGTGGACGTGGTGATGTGTGGGATCGGGCTCAGCGCCTGCACGACAAGATGCAGACAAACCTTTCGATGGCGCGGGATCGTCTCCATTTTCTAGGTAAGTGACCAGTCATATTTCCTATACCCCTAAAACATAGGATTTGTTTACCTCATTTTTTTTTTATTTCATTTTGCACGTTTTCCCCTGCTTTTTTGTTGTCCCCTCCGTTTTGTCCATATGCACATGTTCATCCGGTGCGCAGCTCTGCGTGAGCAGGATTTGCAAATGCAGCGCCTCTCCTTAAAGGAGAAGCAGAAAGAAGAGGCTCAAAGCAAGCCGCAGAAGACCAGGGAGCCCATGCTGGCAGGAATGACCAACGAACCAATGAAACTAAGGGTGCGCAGCAGTGGCTATGGGCCCAAGGCCACCACCAGTGCCCAACCCACTGGTAAGTTCCTCACCAGCTAACGCGCACCCAACAAGATCCTCACACGCCAGCGCAAAGCCACCATAAGCAGATCGATTAGTCAATCCCTATCACCTCACTTGGCACTTGATTTTCTTAGAATTTTTTATTTCGATTGGCCAAATTGTAAACTTGTAAAAGAGTATATTCTTCCGCATTATCCATCTCTGATTGTGCTAATTCGTTCACAGCATCTGGCCGCAAACTGACAATTGGATCCAAACGACCCGTCAACTTGGCCGTGGCCAACAAATCACAAACGCTGCCCCGCAACCTTGGCTCCAAAACATCTGTAGGCGCAGTCCAAAGGCAACCAGCCAAGACAGCTGCAACGCCTCCCGCCGTTCGGCGACAATTTGTGAGTATCTGATAAGTAAACTAAATTTGTTTTTACACATTTGGCAAATACTCAGAAATTAAAATACTAATTAAACTAAATTACTCATATTACAGTCTTCGGGGCGTAATACGCCGCCCCAGCGTTCGCGGACTCCCATAAA

 

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