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CRIMIC CR00404

Gene Taldo

CG2827

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:23940836..23940842	60A12	FBti0199365	78904

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTCCTCTGGATGAAACTGTAGTTCTTCG	GAATAGCGAAATCCAGAAACACCCGTCC

Images

Construct Design Sequences

sgRNA sequence	TATAGTACACTTTACTATAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCGACCCAAAATGTTCAGCG	ACACACGTAAGCACCAAAGG	ATGTGACCGCTATAGTAAAGTGT	CCTGATGCCCTCGGAAAGTT

 

Amplicon 5'

Amplicon 3'

CCGACCCAAAATGTTCAGCGCTTTCTGTACAAGAACGAAAGTATGCACTTATTTATTCGTCCAATTACAAGTAGATGGTTATTTATATTTAACAAGCTATTCACCTAAGATTAGCTTGAATACCTTATCATCACAGTTAAAGATTAATGTTAGGGAGCTAGTTTAAGTCTGCTTAATTGTCAGGAATCCCCCTGATGGCCCCTTATATCGATTCCTAAACCCAAGTTGGGGCTGTTCAGTATTTTTCCAGAGCCGGGCGGTATAAATTGCCGCTCCAGTGGCGGTCTTATTGCATTGAAGTTGGGCAATGGGAAGTGATCGCACGTTAAAAAAGCAAAAGATGTCGGTATTACAGGAACTCAAGAAAATCACCACAATTGTGGCTGACACCGGCGACTTTGAAGGTAAGTACGCACGCAAGGTATTTTTCTTAACAAGATGAGTCCGATTTCAAAGCTAGCGTAAGTTTGCCTCAAAAGTGGGAATCCAGAAATGCAACTAAAGCGAAATGCGAGTCTGAGCTCGAACATCCATACATATATAGAGCATACATAGAATCCGAGTCCAAAGATACATAGGATACATGGATTTATCCAAAAGATAAACATTAACGACGGGCCATCGGATCCGTCGTGTCTGCCGCAGTGAAGATCAAATAAAAGTGAAAAGCAAATCCGAGCTGACTTTAGTTTGACCTTTGGTGCTTACGTGTGT

ATGTGACCGCTATAGTAAAGTGTACTATAGAATGCCACCAGAGATAAGGTTTCGGAGCAGAGTGCCAAACCTGCTCAGACATTTGAAAAGTTCAAAGATATTTTCGGTCGGGCGATTGAAAAATGAAAGTCGCTCAACAGGTTTCGAGGTCTTTCTAAAATTCGCAATGTAATTTGGCCCCGCACTTATGCAAATACTTTACACATTGCTTCCAGCCATCAACATCTATAAGCCCACGGATGCCACCACTAATCCCTCCCTCATCTTGTCCGCTTCGTCCATGGAGCGGTACCAGCCGCTGGTCCAAAAGGCGGTGGAATACGCCAAGGGCAAGAAGGGGTAAGCAGTTCACTTGGGTGTTTGCCACACAAAGCTACATAGAATACCATCGTTTGCAGCTCCGTGAGTGAGCAGGTGGCCGAGGCCATGGACTACCTGTGCGTGCTGTTCGGAACGGAAATCCTGAAGGTTGTGCCCGGTCGCGTTTCCACCGAGATCGATGCCCGCTTGTCCTTCGACACCAAGAAGAGCGTGGAGAAGGCCCTGAAGCTGATCGCCCTGTACAAATCCCTGGGCGTCGACAAGGAGCGAATTCTGATCAAGCTGGCGTCCACCTGGGAGGGCATCAAGGCGGCCGAGATCCTGGAGAACGAGCACGGTGTGCACTGCAACCTGACGCTCCTTTTCTCGTTCGCACAGGCCGTGGCTTGCGCCGAGGCCGGCGTGACCCTCATCTCCCCGTTCGTGGGCCGCATCCTGGACTGGTACGTGGCCAACACGGATACCAAGAAGTTCGAGGCGCTCAAGGACCCGGGCGTGATCTCCGTGACGAACATCTACAACTACTACAAGAAGTTCGGCTACAAGACCCTGGTCATGGGCGCCTCCTTCCGTAACGTGGGCGAAATCAAGGCCCTGGCTGGCTGCGATCTGCTTACCATCAGCCCAGCGCTGCTTAAGGAGCTGGAGAACGAGACCGAGTCGGTGGTCACCTACCTGTCCGTAAGCAACGCCAAGCTGCAGGACATCGAGAAGATCACCGTCGATGAGAGCCGGTTCCGCTGGCTGCTCAACGAGGACGCCATGGCCACCGACAAACTTTCCGAGGGCATCAGG

 

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