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CRIMIC CR00405

Gene CG7766

CG7766

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:9098526..9098542	8C9	FBti0199580	79254

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TTTATTGCCGGTTTTGGGCAGCTGCTTC	TCCTCCACTTCTGTGTCCGCGAAATT

Images

Construct Design Sequences

sgRNA sequence	GAGCGCCGCTTAAATACTACGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CATGGTGGTGGGACAATCGA	CGAGCATCCCAACCAGATCA	AGGAGCGCCGCTTAAATACT	ATCAACGCTCTGCTCTGGAC

 

Amplicon 5'

Amplicon 3'

CATGGTGGTGGGACAATCGAGGACAGGCGACGTTCCGTCAGCGGAGAAGGTCCCTCCATGCCCAGGTTATCAGCTGAAATACGCAAAAAAAAAAAACAGCATTAGTATTCAGTACTACGATGTGGTATCAAAAGCAATCAATCACTCACAATCCACGTTGATGGAGCGCGTCTTCTTGATGGCTCCCTTGCAGGACATGCGTCGCCTCAATGTTCGCGGACGCAATTGTCCGCCGCGCAGATTCATGGTGCTGCGATTGCTAAACGAGCGCAGCAGATGCTCATCCAAATAGGTCTGCACATCCGGATGCAGGCGGTCCGGATAGCCATCCTCGGTGCTGCCCAAAAAGCTCAGATCCGTGATGGCCGAGGTGTTGAGAAAGTCCTTCAGGCTGCCCAGCATTACGCGTGTGCCATTGATGTAGCCCGATTTGAGTTTCCTCATCGTCTGGATCATGGCCAGCGGGATCTTGTCCTGATCTGTGAAATTCCGCAGCGCATCCAATACATTTGTGTTAGCTTTTCGGTTTTTCACATTGTGTTCGGTTCATAGCAATGAATATGTGGGTCTCATGATCTGGTTGGGATGCTCG

AGGAGCGCCGCTTAAATACTACGGGTTAGTCACACAAAAAAACAACATAGTAACCGATAGATAGAAGCACACGATTCCAGCCACCATTGGCAATTAGTCTGCGTTTCGCATTTTCAGTTAGGTGCACAAGGCAAATGCAGTGCAAATACTTAAGGAAATGTTTTAATCACAGGTTGGGGTTATTTACGCAAAGTAGTAAATAAAGTTATTAGCATTAAAGTAGCACTGTTTTTTTTTGGAATATGTCCGTTTTCGGTCTGTGGCGTAATAGTGTATAGATGATAGCTCAGAAGTCCAGAAATCCCAGGATAGCTTAGGCGTTTTTAGCACAGCAGCCAAAATGGCTATATCTTGTGGAAAATGATTGAAAACTAATGGAAGATTGCAAGCTCTATAATTTGTTTTGATCTAAAGATGATTTTCACAAGATATTGGTGTATATAGAGTGTAGAGGTGTTTAAAGCATAGTATTTACACTGAGAAAAGCGAGCAGAAAGAGCGGAAGCAGTCAGGCCATTTTTTACGAAGCAGACACAAGCGGGGGTCACAGGTCACAGATCAAAGATCACAGATCGTAATATCAATTGTTTGGCTTTTTTTTTGGTGTGTGTGTGCAGGAAGGTACATATATGTATATATATGTAATGCAGTGGATTTCTTGGAATCGAGTGTTTTTTGTTTTGTTTTTTTTTTTTTCCATTTTTGGGTGCAAAGTGGGAATCGGATTTCGATTTGGGATCGGGTAGTTTTTTTTTTTATTTCGGGTAGTTACCTAGCATATAGTGGGTTGCCATTAGTGTGATTGTCGGCCTGCCCAACATGTGACGCCAATTGTTGGTCAAGAATGCTAAATTTGTGGTAAAATTGCTGGCAAGCAAATCGGGATCGCGTGTTGTATAATATTCTTCATAGTCGATATGCTGTGTATTTATGGTTGTTGTTGTTGTTGTTGGTGTTGCAGTTGCAGTTGTTGTTGTTGTGTATATTTATTATATTTATTTTGGGATAAAATAAAGAGAAGAAAGGATGTTTTTTGATTTTGATTTTCATTCTCAATTTTCTTTCAACAATTTCATTGGCCAATTTTCGGAGATTTCGGGGGCATGGGAATGAAAATTATAGGGTAATACAAGTCCAGAGCAGAGCGTTGAT

 

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