CR00406

CRIMIC CR00406

Gene muc

CG5261

Information

Donor Location Cytology FBti Stock number
pM37-p12L:7428836..742883627F4FBti0200551Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band short band ATGCGTGATAGTGTTTCGGCTGGCTGAT GCATGTTGACCTCCTTGAAGCTGCATAG

Images

Construct Design Sequences

sgRNA sequence TAACGGAACAATTGGACAAAGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTCCGGAACACATCAGGGTG AGGGCGCTATATGAAAAGCTGT GCGCCCTTTGTCCAATTGTT CGTGGGGCTGAAGTTTGTTG
 
Amplicon 5' Amplicon 3'
TTCCGGAACACATCAGGGTGCCACTGCCCGCACTTTCCCCGACTATGGAGCGCGGATCGATTGTCAGCTGGGAGAAGAAGGAGGGCGATAAGCTCAACGAAGGTACGTGTGATAGTTACCAAAATATCCGAGTTTCAGGATCAAATTTATGACACCCTGATTGCAGGTGACTTGCTGTGCGAGATTGAGACGGATAAGGCCACCATGGGCTTTGAGACGCCCGAGGAGGGCTTTCTGGCTAAGATTCTCATCCAGGGCGGCACCAAGGACGTTCCCGTTGGCCAGCTGCTGTGCATTATCGTGCCGGACCAAGGCAGCGTAGCTGCTTTCGCGAACTTCAAGGACGACGGTGCCGCCGCAGCACCAGCTGCTCCGGCAGCAGCTCCAGCTCCAGCACCGGCAGCAGCAGCAGCTCCGCCACCACCACCACCACCCGCCGCAGCTCCAGCAGCCGCTGCGCCTCCACCAGCTCCCGCCGCTGCTCCAGCTGCAGCAGGTACGGGCCGTGTATATGCCAGTCCCATGGCCAAGCGACTAGCCGAGGCACAACAGCTGCGTCTACAAGGTTGTTTAATCCTAACTAATGCCTCTCTTACACACATCCTGTTCCATTTCGCAATGCATGCATCACCTGACTAACATCCATCATAATCATTCGTTACAGATAACAAAACCTCCCATATACACTATCCAACCGTACAGCTTTTCATATAGCGCCCT GCGCCCTTTGTCCAATTGTTCCGTTACTAATCCGCGGCTTTTGTGTGCAGGCAAGGGCAGTGGAGTCCATGGCTCAATTAAATCTGGTGATCTTGCAGGCCAGAAAGCAGCAGCCAAGCCAGCTGCTGCGGCACCTGCCAAGGCCCCCAGGGCAGCTGGAGCGCGCTACGAGGACATTCCGGTGACCAACATGCGCGCAGTGATTGCCAAACGTCTGCTGGAGTCCAAGACACAACTGCCTCACTACTACGTCACCGTGCAATGCCAAGTGGATAAGGTGGGTTATCACGAACAGATATACTTCTTAATTCAAATTTAATTTAAATGAAATTTTTACAGCTGCTGAAGTTCCGCGCGAAGGTGAACAAGAAGTACGAGAAGCAGGGTGCCCGCGTCTCTGTAAACGACTTCATTATCAAGGCCGTGGCCATTGCCAGTCTTAAGGTTCCCGAAGCGAACTCCGCCTGGATGGACACAGTAATTCGCAAGTACGACGACGTCGATGTTTCGGTTGCTGTCTCCACAGACAAGGGTCTGATTACCCCGATTGTTTTCAATGCCGACCGCAAGGGTGTCCTGGAGATCTCTAAGGATGTCAAGGCGCTGGCAGCCAAGGCGCGCGACAACAAACTTCAGCCCCACG
 
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